Effect of organochlorine contaminants and individual biological traits on blubber retinoid concentrations in bottlenose dolphins (Tursiops truncatus)

Here we assessed retinoids as biomarkers of contaminant exposure by studying whether the sex, age, lipid content and organochlorine concentrations of bottlenose dolphins induced variation in retinoid status and its deposition in blubber. Blubber samples were collected from 47 individuals of known age and gender from Sarasota Bay in June 2000 and 2001. The sample included a representative cross-section of the resident dolphin community, with ages ranging from 2 to 50 years. Organochlorine levels showed the age- and sex-related variation commonly observed in other species, with concentrations increasing in youngsters of both sexes and in adult males, and decreasing in adult females after the onset of maturity. Blubber lipid content was low in the overall population and significantly decreased with age in adult males. Retinoid blubber concentrations were comparable to other odontocete species previously studied, and were strongly determined by lipid content. As a consequence of the latter, retinoid concentration was observed to decrease with age in adult males. This effect could not be statistically dissociated from the negative correlation observed between levels of organochlorines and retinoid blubber concentration. Consequently, we could not clarify whether high organochlorine loads in this population lowered retinoid concentrations or, conversely, whether depleted lipid reserves were indeed responsible for the high organochlorine concentrations and the low retinoid levels detected in blubber. With the current knowledge, both options should be considered and investigated, with initial focus on male dolphins.     

Organochlorine compounds and stable isotopes indicate bottlenose dolphin subpopulation structure around the Iberian Peninsula

Isotopic signatures and organochlorine pollutant loads of organisms reflect the characteristics of the waters in which they live and feed. To investigate population structure of bottlenose dolphins around the Iberian Peninsula we determined delta(13)C and delta(15)N in the skin and organochlorine (OC) levels in the blubber of stranded bottlenose dolphins inhabiting the Mediterranean (Catalonia, Valencia and Balearic Islands) and adjacent Atlantic waters (Huelva and Portugal). OC levels were high in all regions, reflecting the predatory habits of the species, its coastal distribution and the existence of intense agricultural and industrial activity throughout the region. PCB congeners showed a gradient from the relatively more chlorinated forms to those that are less so, and followed a northeast to northwest direction across the Iberian Peninsula. This suggests that PCB inputs are more recent in the temperate latitudes of the eastern Atlantic Ocean than in the western Mediterranean Sea. Comparatively, OC ratios and isotopic signatures proved to be more efficient ways of discriminating groups than did raw OC concentrations. Significant differences in delta(13)C and in PCB congener profiles indicate that dolphins from the Atlantic and the Mediterranean do not intermingle. In addition, the two Atlantic groups differed in delta(15)N signature, tDDT concentration, DDT/PCB ratio and the PCB congener profile, which also suggests some degree of isolation between them. In the Mediterranean, dolphins from Catalonia and Valencia were indistinguishable, suggesting a common distribution area. However, dolphins from the Balearic Islands differed from those of the Peninsula in their DDT/PCB ratio and from all the other sample groups in their PCB congener profiles, which supports the hypothesis that the deep waters between the Islands and the Peninsula represent an effective barrier for the species.     

Trace metals and radionuclides in macroalgae from Moroccan coastal waters

Macroalgae species Codium sp, Bangia atropurpurea, Membranoptera alata, Plocamium cartilagineum, Dictyota dichotoma, Fucus spiralis and Stypocaulon scoparia were collected from seven stations along the north coast of Morocco. Samples were analysed to determine activities of naturally occurring radionuclides ((210)Pb, U isotopes and (40)K) and concentrations of metals (Zn, Fe, Co, Cu, Ni, Mn, Pb, Cd, As and Cr) using radiometric and ICP-OES techniques, respectively. Metal concentrations were within ranges reported in the scientific literature, and concentrations of bio-essential elements were in the order Mn>Fe> Zn>Cu in all samples. Brown algae had the highest concentrations of almost all metals, and concentrations decreased in the order brown>red>green algae. With respect to radionuclides, the red alga P. cartilagineum had the highest activities of (210)Pb, in most cases an order of magnitude higher than for the green alga Codium sp. (234)U and (238)U activities in all algae samples were in the range 0.96- 7.61 and 1.16-6.14 Bq/kg dry weight, respectively. Our analyses of radionuclide activities and metal concentrations in marine macroalgae showed large differences among taxa. These results provide insights into which algal species should be used for biomonitoring programmes.     

Organochlorine contaminant and retinoid levels in blubber of common dolphins (Delphinus delphis) off northwestern Spain

The effect of age, sex, nutritive condition and organochlorine concentration on blubber retinoid concentrations was examined in 74 common dolphins incidentally caught off northwestern Spain. Age and blubber lipid content were strong determinants of the retinoid concentrations in males, while these variables did not account for the variation found in females. Retinoids were positively correlated with organochlorines in males and negatively in females. However, pollution levels were moderate and likely to be below threshold levels above that a toxicological response is to be expected. Thus, a cause-effect relationship between organochlorine and retinoid concentrations could not be properly established, and the observed correlation may be the result of an independent association of the two variables with age. Further research on the influence of the best predictor variables on retinoid dynamics is required to implement the use of retinoids as biomarkers of pollutant exposure in cetaceans.