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Baumann Matthias Israel Christoph Piquer-Rodríguez María Gavier-Pizarro Gregorio Volante José Norberto Kuemmerle Tobias 《Regional Environmental Change》2017,17(4):1179-1191
Regional Environmental Change - The dry forests of Latin America are among the most dynamic deforestation frontiers in the world and are important carbon and biodiversity reservoirs. Our knowledge... 相似文献
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Dr M. Bergere J. Selva M. Baud M. Volante B. Martin J. N. Hugues F. Olivennes R. Frydman M. Auroux 《黑龙江环境通报》1995,15(9):835-841
We performed fluorescence in situ hybridization (FISH) with a chromosome 18-specific probe on human abnormal cleaved embryos, fertilized either by two spermatozoa and exhibiting three pronuclei (3 PN) or normally fertilized and exhibiting two pronuclei (2 PN) with subsequent severe fragmentation and/or blocking. The aim of the study was to evaluate the incidence of chromosome 18 anomalies among these embryos, in order to evaluate the FISH efficiency on such material and to obtain more precise and complete data than those obtained with classical cytogenetic analysis. For the 3 PN cleaved embryos, FISH confirmed the frequent regulation towards diploidy (25 per cent) and the high frequency of mosaics (53 per cent). For the 2 PN blocked or damaged embryos, FISH permitted chromosome evaluation, which was otherwise impossible with classical cytogenetic techniques: we also found a high mosaic frequency (45 per cent) with these embryos. If this frequency were the same for normally developing embryos, it would be a major obstacle to the reliability of either chromosomal or genetic preimplantation diagnosis. 相似文献
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