High serum S-equol content in red clover fed ewes: the classical endocrine disruptor is a single enantiomer

An expedient high performance liquid chromatography (HPLC) method was developed for the quantitative analysis of environmental estrogenic isoflavonoids, particularly (S)-equol, in red clover fed ewes. We report here the phytoestrogen analysis of red clover silage and the serum of ewes fed with this feed. Ewes received daily 10.5 g of formononetin on average from red clover silage. In the serum samples a major part of formononetin had been metabolised to equol (average 7.7 μg ml⁻¹). According to chiral HPLC analysis, the equol present in the serum of ewes was solely of the (S)-form. We also demonstrate the presence of the metabolite O-demethylangolensin (O-DMA) in ovine serum.     

Indigenous knowledge informing management of tropical forests: the link between rhythms in plant secondary chemistry and lunar cycles

This research used knowledge of the indigenous practice of timing nontimber forest product harvest with the full moon to demonstrate that chemicals controlling the decomposition rate of foliage fluctuate with the lunar cycle and may have developed as a result of plant-herbivore interactions. Indigenous knowledge suggests that leaves harvested during the full moon are more durable. Palm leaves harvested during the full moon had higher total C, hemicellulose, complex C and lower Ca concentrations. These chemical changes should make palm leaves less susceptible to herbivory and more durable when harvested during the full moon. This study proposes a mechanism by which plants in the tropics minimize foliage herbivory and influence the decomposition rates of senesced leaves and their durability, especially during the full moon. This research supports the need to use natural life cycles in managing forests and provides a scientific basis for an indigenous community's harvesting practice.     

Dispersion and Reinforcing Potential of Carboxymethylated Nanofibrillated Cellulose Powders Modified with 1-Hexanol in Extruded Poly(Lactic Acid) (PLA) Composites

Bionanocomposites of poly(lactic acid) (PLA) and chemically modified, nanofibrillated cellulose (NFC) powders were prepared by extrusion, followed by injection molding. The chemically modified NFC powders were prepared by carboxymethylation and mechanical disintegration of refined, bleached beech pulp (c-NFC), and subsequent esterification with 1-hexanol (c-NFC-hex). A solvent mix was then prepared by precipitating a suspension of c-NFC-hex and acetone-dissolved PLA in ice-cold isopropanol (c-NFC-hex_sm), extruded with PLA into pellets at different polymer/fiber ratios, and finally injection molded. Dynamic mechanical analysis and tensile tests were performed to study the reinforcing potential of dried and chemically modified NFC powders for PLA composite applications. The results showed a faint increase in modulus of elasticity of 10?% for composites with a loading of 7.5?% w/w of fibrils, irrespective of the type of chemically modified NFC powder. The increase in stiffness was accompanied by a slight decrease in tensile strength for all samples, as compared with neat PLA. The viscoelastic properties of the composites were essentially identical to neat PLA. The absence of a clear reinforcement of the polymer matrix was attributed to poor interactions with PLA and insufficient dispersion of the chemically modified NFC powders in the composite, as observed from scanning electron microscope images. Further explanation was found in the decrease of the thermal stability and crystallinity of the cellulose upon carboxymethylation.     

Uncertainty in Agricultural CH<Subscript>4</Subscript> AND N<Subscript>2</Subscript>O Emissions from Finland – Possibilities to Increase Accuracy in Emission Estimates

According to the United Nations Framework Convention on Climate Change (UNFCCC) and Kyoto Protocol under it, industrial countries have to estimate their greenhouse gas emissions annually, and assess the uncertainties in these estimates. In Finland, agricultural methane (CH₄) and nitrous oxide (N_2O) emissions represent 7% of anthropogenic greenhouse gas emissions, and globally the share is much higher. Agriculture is one of the most uncertain emission categories (representing over 20% of greenhouse gas inventory uncertainty in Finland), due to both high natural variability of the emission sources and poor knowledge of the emission-generating processes. In this paper, we present an uncertainty estimate of agricultural CH₄ and N₂O emissions from Finland in 2002. Uncertainties were estimated based on measurement data, literature and expert judgement, and total uncertainty in agriculture was calculated using Monte Carlo simulation. According to the calculations, agricultural CH₄ and N₂O emissions from Finland were 3.7 to 7.8 Tg carbon dioxide (CO₂) equivalents, 5.4 Tg being the mean value.Estimates of CH₄ emissions are more reliable than those of N₂O. N₂O from agricultural soils was the most uncertain emission category, and the uncertainty was not reduced by using available national measurement data of N₂O fluxes. Sensitivity study revealed that the uncertainty in total agricultural inventory could be 7% points lower, if more accurate emission estimation methods were used, including 1) improved data collection in area estimates of organic soils, 2) climate-specific methods for N₂O from agricultural soils as already presented in literature, and 3) more detailed CH₄ estimation methods for enteric fermentation which can be achieved by investigating national circumstances and digestible systems of animals in more detail.     

提供给热带森林管理的地方知识:植物次生化学节律和月亮周期的关系

本研究运用满月时收获非木材林产品的当地实践如识,来证明控制叶片分解速率的化学物质随月亮周期而波动,并且这种现象可能已发展为一种植物一食草动物之间的相互关系.当地知识认为,满月期收获的叶片更为耐用.满月期收获的棕榈叶片具有较高的总碳量、半纤维素和碳化合物、而钙的浓度则较低.这些化学上的变化使棕榈叶在满月收获时对食草动物不太敏感且更为耐用.本研究提出了热带植物减少食叶功物食用及影响衰老叶片的分解速度和耐用性(尤其在满月期)的作用机制.本研究认为森林经营中有必要利用天然的生命周期,并为当地人的收获实践提供了科学依据.     

Concentrations of (137)Cs in summer pasture plants that reindeer feed on in the reindeer management area of Finland

Samples of summer pasture plants that reindeer feed on were collected in order to study ¹³⁷Cs concentrations in different plant species and in species nested in certain site types, and to study the regional distribution of ¹³⁷Cs in the Finnish reindeer management area. Plant species were categorized by the site types of mineral soil forest (xeric heath forest and mesic heath forest) and peatland. A third category called ’other plant species’ included plants with various site types, poorly determined species and species with poor statistics. The ¹³⁷Cs concentrations in different site types differed significantly. The mean ¹³⁷Cs concentrations of the whole reindeer management area in the xeric heath forest plant species was 44 ± 27 Bq/kg dw, in the mesic heath forest plant species 75 ± 59 Bq/kg dw and in the peatland plant species 219 ± 150 Bq/kg dw. The peatland species uptake ¹³⁷Cs more efficiently than plant species of mineral soil forests. A particularly efficient collector of ¹³⁷Cs was Trichophorum sp. It is suggested that Trichophorum sp. could be used as an indicator species for reindeer summer fodder plants. The highest concentrations of ¹³⁷Cs were found in Southern Lapland and the lowest in Northern Lapland. Today, the concentrations of ¹³⁷Cs in summer pasture plants that reindeer feed on in Finland are at such a level that there is no need to avoid any plant species. In the case of future nuclear fallout, reindeer grazing in peatlands would increase concentrations of ¹³⁷Cs in reindeer meat.     

New potential mammalian lignan metabolites of environmental phytoestrogens

Seven new dibenzylbutyrolactone and six new dibenzylbutanediol lignans were prepared to assist in the identification of a number of potential mammalian metabolites of the endocrine disrupting plant lignans syringaresinol and medioresinol, present in food.     

Designing conservation-development policies for the forest frontier

The conservation of forests in expanding frontier landscapes is critically important to maintain intact forest ecosystems and support forest dependent communities. To conserve frontier forests, policy approaches are needed that conserve forests and advance the well-being of local resource dependent communities. To identify such approaches, the forest conservation and development framework (FCDF) was designed to find place-based conservation-development policies that target system leverage-points influencing land-use practices. To demonstrate the utility of the FCDF, a portfolio of conservation-development policies were identified for Peru’s Manu-Tambopata Corridor (MAT) and evaluated by local land-users. Results of the MAT case study show high levels of interest in the proposed policies, but a wide variation in interest levels relative to personal circumstances and policies proposed. Barriers to implementing conservation-development policies in frontier environments were also identified, including high value land-use alternatives generating high opportunity costs for accepting PES payments (e.g., REDD+), insecure land tenure and conflicting authorizations limiting land-users policy choices, and broad demographic diversity among local land-users. Collectively, this research suggests the FCDF is a useful approach for identifying policies matched to local conditions that advance conservation and human development. This research also indicates policy design in frontier environments is most effective when adapted to local conditions, seeks to identify a mix of complementary policies, and is targeted at key system variables influencing land-use practices (i.e., system leverage-points). Importantly, the MAT case study also highlights how even in rapidly changing frontier landscapes, land-users are interested in policies that advance conservation and development goals.