低浓度臭氧在含钴盐硅胶上的吸附研究

常压下对含钴盐硅胶进行低浓度臭氧的吸附实验，吸附达到饱和后用氮气作为载气加热解吸。结果显示，含钴盐的硅胶对臭氧具有较强的吸附能力；臭氧解吸量随着解吸温度的上升而增加；吸附了臭氧的含钴硅胶粒子在常温(313．15K)下可在60min内不问断解吸出臭氧。     

Inorganic iodine incorporation into soil organic matter: evidence from iodine K-edge X-ray absorption near-edge structure

The transformation of inorganic iodine (I⁻ and IO₃⁻) incubated in soils with varying amounts of organic matter (Andosols from the surface layer of an upland field and forest, as well as Acrisols from surface and subsurface layers of an upland field) was investigated by using the iodine K-edge X-ray absorption near-edge structure (XANES). After 60 d of reaction, both I⁻ and IO₃⁻ were transformed into organoiodine in surface soils containing sufficient amounts of organic matter, whereas IO₃⁻ remained unchanged in the subsurface soil of Acrisols with low organic matter contents. Transformation of IO₃⁻ into organoiodine was not retarded when the microbial activity in soil was reduced by γ-ray irradiation, suggesting that microbial activity was not essential for the transformation of inorganic iodine into organoiodine. Soil organic matter has the ability to transform inorganic iodine into organoiodine.     

Postcopulatory mate guarding by vocalization in the Formosan squirrel

The Formosan squirrel, Callosciurus erythraeus thaiwanensis, emitted different vocalizations in response to terrestrial and aerial predators and snakes. Each vocalization caused nearby individuals to adopt a different type of anti-predator behaviour. In mating bouts, males produced two types of loud calls: precopulatory calls, emitted before copulations, and postcopulatory calls, emitted after copulations. The latter continued for 17 min on average. The estrous female and other males attending the mating bouts stopped moving during the postcopulatory call, so that the calling male was able to tend the female without interruption. The sound characteristics of anti-terrestrial-predator and postcopulatory calls recorded in the captivity were compared, and none of the ten characters of duration and frequency measured differed between the two calls. Playback experiments also showed that responses to the sounds in two different contexts, escape behaviour and defensive immobility, did not differ. The similarity between anti-predator and postcopulatory calls is discussed with reference to the possibility of manipulation and other explanatory hypotheses.     

Evaluation of the toxicity of tetrabromobisphenol A and some of its oxidation products using a micro-scale algal growth inhibition test

A micro-scale algal growth inhibition (μ-AGI) test using a common micro-plate based fluorometric detection was used to demonstrate the effects of humic substances (HSs) on the toxicity of tetrabromobisphenol A (TBBPA) and its oxidative decomposition products 2,5-dibromo-1,4-benzoquinone (2,5-DBBQ), 2,5-dibromohydroquinone (2,5-DBHQ), 2,6-dibromobenzoquinone (2,6-DBBQ), and 2,6-dibromophenol (2,6-DBP) to Pseudokirchneriella subcapitata. The EC₅₀ values were: EC_50(TBBPA) = 7 mg L^?1, EC_50(2,5-DBHQ) = 7 mg L^?1, EC_50(2,5-DBBQ) = 19 mg L^?1, EC_50(2,6-DBP) = 49 mg L^?1, and EC_50(2,6-DBBQ) = 13 mg L^?1. The toxicity of the chemicals was slightly lower in the presence of HA. The toxicity of TBBPA decomposed by a biomimetic catalytic system consisting of iron (III) 5,10,15,20-tetrakis (p-sulfonatophenyl) porphyrin (Fe(III)-TPPS) and KHSO₅ was also evaluated using P. subcapitata and Chlamydomonas reinhardtii.     

Comparison of the changes in temperatures among rural, urban and metropolitan areas around the Inland Sea in Japan

We investigated the changes in temperatures in Takamatsu, Tadotsu and Osaka areas in Japan. Data of temperatures in Takamatsu (urban area: 1942-2009), Tadotsu (rural area: 1893-2009) and Osaka (metropolitan area: 1883-2009) areas around the Inland Sea in Japan was obtained from Japan Meteorological Agency. The changes in temperatures in three areas in Japan were analyzed and compared. By using data from 1942 to 2009, various parameters of temperatures were significantly correlated with years. The changes in mean temperature in August and in a year in Takamatsu area for 50 years were highest (August: 1.6°C, Year: 1.8°C) among three areas. In addition, correlation coefficient rate between years and the number of days over the level of 34°C in August in Takamatsu area was highest (r = 0.567, p < 0.0001). Parameters of temperatures were gradually increased with years in all three areas. The heat island effect in Takamatsu area was similar to Osaka area in Japan.     

Behavior of di(2-ethylhexyl) phthalate discharged from domestic waste water into aquatic environment

The behavior of di(2-ethylhexyl) phthalate (DEHP) discharged from domestic waste water into river water, sediment and submerged aquatic vegetation was investigated. The concentrations of DEHP were found to be between 8-25 microg L(-1) in river water, 1,000-2,000 microg kg(-1) in sediment and less than 20-2,000 microg kg(-1) in submerged aquatic vegetation. The experiments performed in laboratory were on the biodegradation of DEHP in water and sediment, and also adsorption equilibrium of DEHP between water and sediment. The results obtained from the investigations made it clear that the high enrichment of DEHP from water to sediment was caused from not only its high adsorptive potential but also slow degradation in sediment.     

Beneficial utilization of a tuna processing by-product as fish-feed additive

During tuna processing for human consumption, the fish are initially boiled in water, creating ~7000 tons of wastewater a year in Tosashimizu City, Japan. The wastewater (tuna broth; TB) is rich in free amino acids and peptides; therefore, we investigated two methods of utilizing it beneficially. In experiment 1, crude TB was used as a growth enhancer for two farmed species, juvenile yellowtail (Seriola quinqueradiata) and red sea bream (Pagrus major). Juveniles of both species, fed commercial extruded pellets mixed with 300 mL TB, showed significantly better growth than the unsupplemented control group. In experiment 2, concentrated TB was used as a dietary ingredient for juvenile yellowtail to improve palatability and growth. Dietary preferences and growth were rated as follows: fish meal diet > soy protein concentrate (SPC) + TB diet > SPC + krill meal diet > SPC diet. The beneficial effects of TB as a dietary supplement were evident. Crude TB enhanced juvenile growth at minimal cost. Concentrated TB requires processing, with associated costs; however, it proved to be a valuable fish-feed supplement because of its high palatability and growth-promoting effect. The utilization of TB also contributes to reducing the environmental impact of fish wastewater.     

Determination of PCDD/Fs and dioxin-like PCBs in fish oils for feed ingredients by congener-specific chemical analysis and CALUX bioassay

The present research was intended to determine the suitability of the CALUX assay as a screening method for dioxins in fish oil used as a feed ingredient in Japan. Alteration of TEQ in fish oil according to newly proposed toxic equivalency factors (TEF) is also discussed. In the analysis, polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) in 41 fish oil samples were determined by using high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS) and CALUX bioassay. The mean TEQ values derived from 1998 WHO-TEF of PCDD/Fs and DL-PCBs were 2.6 and 9.9 pg g(-1) (ww), respectively. The levels of TEQ derived from the recently re-evaluated 2005 WHO-TEF were slightly lower than those of the former in both groups. Notably, the contribution of mono-ortho DL-PCBs to total 2005 WHO-TEQ was considerably decreased compared to the case of 1998 WHO-TEQ, resulting from the reduction in its TEF values, while the non-ortho DL-PCBs contribution was increased. The mean TEQ determined by CALUX assay for PCDD/Fs was approximately three times higher, whereas DL-PCBs was approximately two times lower than WHO-TEQ determined by HRGC/HRMS; the sum of PCDD/Fs and DL-PCBs was very similar by both methods. The correlation coefficients of TEQ between the CALUX assay and HRGC/HRMS analysis were 0.84, 0.89, and 0.90 for PCDD/Fs, DL-PCBs, and the sum, respectively. These results suggest that the CALUX assay is a very useful method for the screening of dioxin-related compounds in fish oils.     

Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish

To analyze the effect of polychlorinated biphenyl (PCB) 118 on fish bone metabolism, we examined osteoclastic and osteoblastic activities, as well as plasma calcium levels, in the scales of PCB (118)-injected goldfish. In addition, effect of PCB (118) on osteoclasts and osteoblasts was investigated in vitro. Immature goldfish, in which the endogenous effects of sex steroids are negligible, were used. PCB (118) was solubilized in dimethyl sulfoxide at a concentration of 10 ppm. At 1 and 2 days after PCB (118) injection (100 ng/g body weight), both osteoclastic and osteoblastic activities, and plasma calcium levels were measured. In an in vitro study, then, both osteoclastic and osteoblastic activities as well as each marker mRNA expression were examined. At 2 days, scale osteoclastic activity in PCB (118)-injected goldfish increased significantly, while osteoblastic activity did not change significantly. Corresponding to osteoclastic activity, plasma calcium levels increased significantly at 2 days after PCB (118) administration. Osteoclastic activation also occurred in the marker enzyme activities and mRNA expressions in vitro. Thus, we conclude that PCB (118) disrupts bone metabolism in goldfish both in vivo and in vitro experiments.