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Dominique M. Poncelet Nicole Cavender Teresa J. Cutright John M. Senko 《Environmental monitoring and assessment》2014,186(3):1917-1929
To assess the microbiological changes that occur during the maturation of overburden that has been disturbed by surface mining of coal, a surface mining-disturbed overburden unit in southeastern Ohio, USA was characterized. Overburden from the same unit that had been disturbed for 37 and 16 years were compared to undisturbed soil from the same region. Overburden and soil samples were collected as shallow subsurface cores from each subregion of the mined area (i.e., land 16 years and 37 years post-mining, and unmined land). Chemical and mineralogical characteristics of overburden samples were determined, as were microbial respiration rates. The composition of microbial communities associated with overburden and soil were determined using culture-independent, nucleic acid-based approaches. Chemical and mineralogical evaluation of overburden suggested that weathering of disturbed overburden gave rise to a setting with lower pH and more oxidized chemical constituents. Overburden-associated microbial biomass and respiration rates increased with time after overburden disturbance. Evaluation of 16S rRNA gene libraries that were produced by “next-generation” sequencing technology revealed that recently disturbed overburden contained an abundance of phylotypes attributable to sulfur-oxidizing Limnobacter spp., but with increasing time post-disturbance, overburden-associated microbial communities developed a structure similar to that of undisturbed soil, but retained characteristics of more recently disturbed overburden. Our results indicate that over time, the biogeochemical weathering of disturbed overburden leads to the development of geochemical conditions and microbial communities that approximate those of undisturbed soil, but that this transition is incomplete after 37 years of overburden maturation. 相似文献
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Disaster Management in the Caribbean 总被引:1,自引:0,他引:1
Jean Luc Poncelet 《Disasters》1997,21(3):267-279
This paper identifies the main trends in approaches to disaster management in the Caribbean at both regional and national levels over the past 20 years. It highlights the main reasons why and how the region has moved from an ad hoc response to an organised approach. Finally, it suggests ways in which future regional and national disaster management could be improved. 相似文献
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