The occurrence of antihistamines in sewage waters and in recipient rivers

Background, aim and scope  Each year, large quantities of pharmaceuticals are consumed worldwide for the treatment and prevention of human and animal diseases. Although the drugs and the metabolites observed in the wastewaters and in the environment are present at concentrations several orders of magnitude lower than the concentrations required to exert their effects in humans or animals, their long-term impact on the environment is commonly not known. In this study, the occurrence of six antihistamines, which are used for the relief of allergic reactions such as hay fever, was determined in sewage treatment plants wastewaters and in recipient river waters. Materials and methods  The occurrence of the antihistamines cetirizine, acrivastine, fexofenadine, loratadine, desloratadine and ebastine in sewage treatment plants wastewaters and in recipient river waters was studied. The analytical procedure consisted of solid-phase extraction of the water samples followed by liquid chromatography separation and detection by a triple-quadrupole mass spectrometer in the multiple reaction mode. Results  Cetirizine, acrivastine and fexofenadine were detected in both influent and effluent wastewater samples at concentration levels ranging from about 80 to 220 ng/L, while loratadine, desloratadine and ebastine could not be detected in any samples. During sewage treatment, the concentration of the antihistamines dropped by an average of 16–36%. Furthermore, elevated concentrations of antihistamines were observed in samples collected during the season of most intensive plant pollen production, i.e. in May. In the river water samples, the relative pattern of occurrence of cetirizine, acrivastine and fexofenadine was similar to that in the wastewater samples; although the concentration of the compounds was substantially lower (4–11 ng/L). The highest concentrations of the studied drugs were observed near the discharging point of the sewage treatment plant. Discussion  The highest concentrations of antihistamines in STP wastewaters correlate with the outbreak of allergic reaction caused by high amounts of plant pollens in the air. The analysis results of the river water samples show that the antihistamines are carried far away from the effluent discharge points. They may account for a part of the mix of pharmaceuticals and of pharmaceutical metabolites that occur downstream of STPs. Conclusions  Antihistamines are poorly degraded/eliminated under the biological treatment processes applied in the wastewater treatment plants and, consequently, they are continuously being discharged along with other drugs to the aquatic environment. Recommendations and perspectives  As a huge quantity and variety of drugs and their metabolites are continuously discharged to rivers and the sea, the compounds should be considered as contaminants that may possess risks to the aquatic ecosystem. Further studies are urgently needed on the environmental fate of the antihistamines and other pharmaceuticals in the aquatic environment. These studies should be concerned with the stability of the compounds, their transformation reactions and the identity of the transformation products, the distribution of drugs and their uptake and effects in organisms. On the basis of these studies, the possible environmental hazards of pharmaceuticals may be assessed.     

Monitoring of symptoms in estimating the effect of intervention in the sick building syndrome: A field study

Employees in six day-care institutions were asked to fill out a simple questionnaire before and one year after technical changes were made in their buildings. The institution were defined as sick buildings, based on a prevalence (exceeding 40%) of irritative symptoms and general symptoms among the employees. Based on the technical measurements there were no obvious reasons for the complaints. It was decided, however, to remove man-made mineral-acoustic ceilings and to install mechanical ventilation. One year later there was a significant reduction in the prevalence of symptoms (irritative and general symptoms related to the sick building syndrome) among the employees. The study indicates that systematic monitoring of employees' symptoms before and after corrective action is an important indicator of the benefit of the actions and might be used routinely by architects, engineers, and local authorities in dealing with indoor climate problems. Follow-up studies might give more knowledge of the causes of sick building syndrome.     

Exposure to radiation from global system for mobile communications at 1,800 MHz significantly changes gene expression in rat hippocampus and cortex

We have earlier shown that radio frequency electromagnetic fields can cause significant leakage of albumin through the blood–brain barrier of exposed rats as compared to non-exposed rats, and also significant neuronal damage in rat brains several weeks after a 2 h exposure to a mobile phone, at 915 MHz with a global system for mobile communications (GSM) frequency modulation, at whole-body specific absorption rate values (SAR) of 200, 20, 2, and 0.2 mW/kg. We have now studied whether 6 h of exposure to the radiation from a GSM mobile test phone at 1,800 MHz (at a whole-body SAR-value of 13 mW/kg, corresponding to a brain SAR-value of 30 mW/kg) has an effect upon the gene expression pattern in rat brain cortex and hippocampus—areas where we have observed albumin leakage from capillaries into neurons and neuronal damage. Microarray analysis of 31,099 rat genes, including splicing variants, was performed in cortex and hippocampus of 8 Fischer 344 rats, 4 animals exposed to global system for mobile communications electromagnetic fields for 6 h in an anechoic chamber, one rat at a time, and 4 controls kept as long in the same anechoic chamber without exposure, also in this case one rat at a time. Gene ontology analysis (using the gene ontology categories biological processes, molecular functions, and cell components) of the differentially expressed genes of the exposed animals versus the control group revealed the following highly significant altered gene categories in both cortex and hippocampus: extracellular region, signal transducer activity, intrinsic to membrane, and integral to membrane. The fact that most of these categories are connected with membrane functions may have a relation to our earlier observation of albumin transport through brain capillaries.     

Maternal transfer of brominated flame retardants in zebrafish (Danio rerio)

In many species reproduction and embryonic development have been shown to be sensitive to environmental contaminants. Understanding embryonic exposure to environmental contaminants is thus highly important. In this study concentrations of brominated flame retardants (BFRs) were measured in zebrafish eggs after parental exposure for 42 days via the diet. Zebrafish were exposed to two doses of eleven structurally-diverse BFRs. Eight BFRs were detected in the female zebrafish and maternal transfer to eggs was evident for all eight compounds. The highest concentrations in eggs were observed for hexabromocyclododecane (HBCD) and 2,4,4'-tribromodiphenyl ether (BDE 28), followed by 2,2',3,4,4',5',6-heptabromodiphenyl ether (BDE 183) and tetrabromobisphenol A 2,3-dibromopropyl ether (TBBPA DBPE). Five potential BFR metabolites were tentatively identified in female fish and maternal transfer was observed also for these compounds. The lipid adjusted concentrations in eggs were significantly higher than the concentrations in female fish for several of the BFRs. Further, the results showed a generally higher transfer in the lower exposure level and also indicated a dependency on the physico-chemical properties of the compounds.     

Energy and carbon balances of wood cascade chains

In this study we analyze the energy and carbon balances of various cascade chains for recovered wood lumber. Post-recovery options include reuse as lumber, reprocessing as particleboard, pulping to form paper products, and burning for energy recovery. We compare energy and carbon balances of chains of cascaded products to the balances of products obtained from virgin wood fiber or from non-wood material. We describe and quantify several mechanisms through which cascading can affect the energy and carbon balances: direct cascade effects due to different properties and logistics of virgin and recovered materials, substitution effects due to the reduced demand for non-wood materials when wood is cascaded, and land use effects due to alternative possible land uses when less timber harvest is needed because of wood cascading. In some analyses we assume the forest is a limiting resource, and in others we include a fixed amount of forest land from which biomass can be harvested for use as material or biofuel. Energy and carbon balances take into account manufacturing processes, recovery and transportation energy, material recovery losses, and forest processes. We find that land use effects have the greatest impact on energy and carbon balances, followed by substitution effects, while direct cascade effects are relatively minor.     

Scenario simulation of water security in China

Umited water resources, increasing demand, low use efficiency, and serious pollution result in severe water resource difficult in China. The evaluation of addressing water problems and the search for effective countermeasures that ensure sustainable water use are key to China‘ s sustainable development. The “compound water security“ consists of food security, life security, environmental security, and economic security. By establishing a conceptual model, the water security of China has been simulated in terms of four scenarios called BAU(the business-as-usual scenario), TEC(the technology and economics scenario), IVL(the institution, values, and lifestyles scenario) and TSD(toward sustainable development) in this paper. The results indicated that water crises, especially water shortages, are being experienced now and will continue to do so for a relatively long time in China and that it is possible to reach a basic balance between supply and demand of water and grain under the TSD developing pattern by a series of approaches including technological innovation, policy adjustments, and behaviour inducement.     

Carbon Dioxide Balance of Wood Substitution: Comparing Concrete- and Wood-Framed Buildings

In this study a method is suggested to compare the net carbon dioxide (CO₂) emission from the construction of concrete- and wood-framed buildings. The method is then applied to two buildings in Sweden and Finland constructed with wood frames, compared with functionally equivalent buildings constructed with concrete frames. Carbon accounting includes: emissions due to fossil fuel use in the production of building materials; the replacement of fossil fuels by biomass residues from logging, wood processing, construction and demolition; carbon stock changes in forests and buildings; and cement process reactions. The results show that wood-framed construction requires less energy, and emits less CO₂ to the atmosphere, than concrete-framed construction. The lifecycle emission difference between the wood- and concrete-framed buildings ranges from 30 to 130 kg C per m² of floor area. Hence, a net reduction of CO₂ emission can be obtained by increasing the proportion of wood-based building materials, relative to concrete materials. The benefits would be greatest if the biomass residues resulting from the production of the wood building materials were fully used in energy supply systems. The carbon mitigation efficiency, expressed in terms of biomass used per unit of reduced carbon emission, is considerably better if the wood is used to replace concrete building material than if the wood is used directly as biofuel.     

Identification of a novel fluorescent adduct formed in the reaction of malonaldehyde with adenosine

Malonaldehyde was reacted with adenosine in aqueous solution at acidic conditions and the reaction mixtures were analysed by HPLC. Four major product peaks were observed in the chromatogram recorded by the UV detector at 320 nm. Two of the peaks could be deduced to the previously characterised malonaldehyde-adenosine reaction product 9-(beta-D-ribofuranosyl)-6-(5,7-diformyl-2H-3,6-dihydro-2,6-methano-1,3-oxazocin-3-yl)purine (M3A) and to the ribose analogue of the 2'-deoxy adduct 9-(2'-deoxy-beta-D-ribofuranosyl)-6-(3,5-diformyl-4-methyl-1,4-dihydro-1-pyridyl)purine (M2AA-dA). The two other peaks were due to previously uncharacterised adducts. Upon isolation of these adduct peaks it was found that the peaks are interconverted to each other, one adduct peak dominating over the other one. On the basis of data recorded by UV, fluorescence and 1H NMR spectroscopy, and mass spectrometry, the structure of the major adduct could be determined as 9-(beta-D-ribofuranosyl)-6-(3,5-diformyl-4-etanal-1,4-dihydro-1-pyridyl)purine (3M-A). The adduct is most likely formed by reaction of adenosine with a malonaldehyde condensation product consisting of three units of malonaldehyde. The highest yield of 3M-A was obtained in the reaction performed at pH 4.6 and 80 degrees C for 75 h. The minor form of the interconverting peaks could not be characterised on the basis of the spectral data. However, it is concluded that the minor peak most likely represents the hydrated form of 3M-A. Since these adducts are formed only in trace amounts at neutral pH and 37 degrees C and the formation requires high amounts of malonaldehyde, it is likely that the adducts are not formed in DNA in vivo and thus not contribute to the malonaldehyde genotoxicity.     

Sex,food and conflicts: nutrition dependent nuptial feeding and pre-mating struggles in scorpionflies

Female and male reproductive interests often differ. In species in which matings are accompanied by a transfer of resources valuable for both participants, such as nuptial prey gifts, conflicts may readily occur. Scorpionflies may use alternative mating tactics. One is to offer a prey item (dead arthropod) to females in exchange for mating. This prey gift tactic includes a conflict because a male must decide on whether to offer the gift rather than to fight the female and consume the gift. The outcome may depend on the nutritional status of both males and females. Males may be more willing to give if they themselves are satiated and the condition of the females may influence the payoff from the males’ investment. Similarly, females may be more willing to accept food gifts if they are in poor nutritional condition. In this study of the scorpionfly Panorpa cognata, I experimentally manipulated the feeding history of both males and females. I observed the outcome of the direct interactions that followed when males that were holding prey were approached by females. I found that well-fed males offered the food gift sooner than males in poor nutritional condition that fed extensively on the food item before offering. Female condition had no significant influence on whether prey items were offered by males or accepted by females. I also found that well-fed males rarely searched for prey to pursue the prey gift tactic in courtship. Thus, the prey tactic does not seem to be the males’ first option.