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ROMSAIYUD Angsana SONGKASIRI Warinthom NOPHARATANA Annop CHAIPRASERT Pawinee 《环境科学学报(英文版)》2009,21(7)
The productivity and efficiency of cellulase are significant in cellulose hydrolysis. With the accumulation of volatile fatty acids (VFAs), the pH value in anaerobic digestion system is reduced. Therefore, this study will find out how the pH and the amount of acetate influence the enzymatic hydrolysis of cellulose. The effects of pH and acetate on cellulase produced from Bacillus coagulans were studied at various pH 5-8, and acetate concentrations (0-60 mmol/L). A batch kinetic model for enzymatic cellulose hydrolysis was constructed from experimental data and performed. The base hypothesis was as follows: the rates of enzymatic cellulose hydrolysis rely on pH and acetate concentration. The results showed that the suitable pH range for cellulase production and cellulose hydrolysis (represents efficiency of cellulase) was 2.6-7.5, and 5.3-8.3, respectively. Moreover, acetate in the culture medium had an effect on cellulase production (K1= 49.50 mmol/L, n=1.7) less than cellulose hydrolysis (K1=37.85 mmol/L, n=2.0). The results indicated that both the pH of suspension and acidogenic products influence the enzymatic hydrolysis of cellulose in an anaerobic environment. To enhance the cellulose hydrolysis rate, the accumulated acetate concentration should be lower than 25 mmol/L, and pH should be maintained at 7. 相似文献
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ROMSAIYUD Angsan SONGKASIRI Warinthorn NOPHARATANA Annop CHAIPRASERT Pawinee 《环境科学学报(英文版)》2009,21(7):965-970
The productivity and e ciency of cellulase are significant in cellulose hydrolysis. With the accumulation of volatile fatty acids
(VFAs), the pH value in anaerobic digestion system is reduced. Therefore, this study will find out how the pH and the amount of acetate
influence the enzymatic hydrolysis of cellulose. The e ects of pH and acetate on cellulase produced from Bacillus coagulans were
studied at various pH 5–8, and acetate concentrations (0–60 mmol/L). A batch kinetic model for enzymatic cellulose hydrolysis was
constructed from experimental data and performed. The base hypothesis was as follows: the rates of enzymatic cellulose hydrolysis
rely on pH and acetate concentration. The results showed that the suitable pH range for cellulase production and cellulose hydrolysis
(represents e ciency of cellulase) was 2.6–7.5, and 5.3–8.3, respectively. Moreover, acetate in the culture medium had an e ect on
cellulase production (KI = 49.50 mmol/L, n = 1.7) less than cellulose hydrolysis (KI = 37.85 mmol/L, n = 2.0). The results indicated
that both the pH of suspension and acidogenic products influence the enzymatic hydrolysis of cellulose in an anaerobic environment.
To enhance the cellulose hydrolysis rate, the accumulated acetate concentration should be lower than 25 mmol/L, and pH should be
maintained at 7. 相似文献
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Rachbordin Wongnoi Warinthorn Songkasiri Chantaraporn Phalakornkule 《Water environment research》2007,79(2):199-207
The objective of this study was to investigate the influence of a three-phase separator configuration on the performance of an upflow anaerobic sludge bed (USAB) treating wastewater from a fruit canning factory. The performances of two 30-L UASB reactors--one with a modified three-phase separator giving a spiral flow pattern and the other with a conventional configuration-were investigated in parallel. Wastewater, with a chemical oxygen demand (COD) concentration between 2000 and 7000 mg/L, was obtained from a fruit-canning factory. Based on the effluent data of the first 100 operation days, the UASB with the three-phase separator giving spiral flow patterns yielded up to 25% lower biomass washout. It also showed better efficiencies in treating wastewater--up to 60% lower effluent COD, up to 20% higher COD percent removal, and up to 29% higher biogas production. This work presents evidence of an improvement on the conventional physical design of a UASB. 相似文献
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