收费全文 | 14840篇 |
免费 | 760篇 |
国内免费 | 5297篇 |
安全科学 | 924篇 |
废物处理 | 952篇 |
环保管理 | 1176篇 |
综合类 | 8233篇 |
基础理论 | 2553篇 |
环境理论 | 2篇 |
污染及防治 | 5386篇 |
评价与监测 | 548篇 |
社会与环境 | 502篇 |
灾害及防治 | 621篇 |
2023年 | 226篇 |
2022年 | 673篇 |
2021年 | 551篇 |
2020年 | 420篇 |
2019年 | 386篇 |
2018年 | 493篇 |
2017年 | 657篇 |
2016年 | 685篇 |
2015年 | 855篇 |
2014年 | 1166篇 |
2013年 | 1627篇 |
2012年 | 1272篇 |
2011年 | 1359篇 |
2010年 | 972篇 |
2009年 | 966篇 |
2008年 | 1026篇 |
2007年 | 937篇 |
2006年 | 839篇 |
2005年 | 607篇 |
2004年 | 423篇 |
2003年 | 555篇 |
2002年 | 489篇 |
2001年 | 413篇 |
2000年 | 436篇 |
1999年 | 476篇 |
1998年 | 425篇 |
1997年 | 359篇 |
1996年 | 339篇 |
1995年 | 286篇 |
1994年 | 238篇 |
1993年 | 188篇 |
1992年 | 151篇 |
1991年 | 89篇 |
1990年 | 69篇 |
1989年 | 55篇 |
1988年 | 47篇 |
1987年 | 30篇 |
1986年 | 25篇 |
1985年 | 13篇 |
1984年 | 15篇 |
1983年 | 18篇 |
1982年 | 11篇 |
1981年 | 12篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1976年 | 2篇 |
1975年 | 4篇 |
1974年 | 2篇 |
1971年 | 1篇 |
1966年 | 1篇 |
The spread of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) has become an increasingly serious global public health issue. This study investigated the distribution characteristics and influencing factors of ARB and ARGs in greenhouse vegetable soils with long-term application of manure. Five typical ARGs, four heavy metal resistance genes (MRGs), and two mobile genetic elements (MGEs) were quantified by real-time quantitative polymerase chain reaction (qPCR). The amount of ARB in manure-improved soil greatly exceeded that in control soil, and the bacterial resistance rate decreased significantly with increases in antibiotic concentrations. In addition, the resistance rate of ARB to enrofloxacin (ENR) was lower than that of tylosin (TYL). Real-time qPCR results showed that long-term application of manure enhanced the relative abundance of ARGs in vegetable soils, and the content and proportion of quinolone resistance genes were higher than those of macrolide resistance genes. Redundancy analysis (RDA) showed that qepA and qnrS significantly correlated with total and available amounts of Cu and Zn, highlighting that certain heavy metals can influence persistence of ARGs. Integrase gene intI1 correlated significantly with the relative abundance of qepA, qnrS, and ermF, suggesting that intI1 played an important role in the horizontal transfer of ARGs. Furthermore, there was a weakly but not significantly positive correlation between specific detected MRGs and ARGs and MGEs. The results of this study enhance understanding the potential for increasing ARGs in manure-applied soil, assessing ecological risk and reducing the spread of ARGs.
Graphic abstract 相似文献Microplastic pollution is occurring in most ecosystem, yet their presence in high altitude clouds and their influence on cloud formation and climate change are poorly known. Here we analyzed microplastics in cloud water sampled at the summits of Japan mountains at 1300–3776 m altitude by attenuated total reflection imaging and micro-Fourier transform infrared spectroscopy. We observed nine microplastics including polyethylene, polypropylene, polyethylene terephthalate, polymethyl methacrylate, polyamide 6, polycarbonate, ethylene–propylene copolymer or polyethylene–polypropylene alloy, polyurethane, and epoxy resin. Microplastic were fragmented, with mean concentrations ranging from 6.7 to 13.9 pieces per liter, and with Feret diameters ranging from 7.1 to 94.6 μm. Microplastics bearing hydrophilic groups such as carbonyl and/or hydroxyl groups were abundant, suggesting that they might have acted as condensation nuclei of cloud ice and water. Overall, our finding suggest that high-altitude microplastics cloud influence cloud formation and, in turn, might modify the climate.
相似文献Animal manure is an important source of antibiotics and antibiotic resistance genes (ARGs) in the environment. However, the difference of antibiotic residues and ARG profiles in layer and broiler manure as well as their compost remains unexplored. In this study, we investigated the profiles of twelve antibiotics, seventeen ARGs, and class 1 integrase gene (intI1) in layer and broiler manure, and the corresponding compost at large-scale. Compared with layer manure, broiler manure exhibited approximately six times more residual tetracyclines, especially chlortetracycline. The relative abundances of qnrS and ermA genes in broiler manure were significantly higher than those in layer manure. The concentration of tetracyclines not only had a significantly positive correlation with tetracycline resistance genes (tetA and tetC) but was also positively correlated with quinolone resistance (qepA, qnrB, and qnrS) and macrolide resistance (ermA and ermT). Most ARGs in manure were reduced after composting. However, the relative abundance of sulfonamide resistance gene sul1 increased up to 2.41% after composting, which was significantly higher than that of broiler (0.41%) and layer (0.62%) manure. The associated bacterial community was characterized by high-throughput 16S rRNA gene sequencing. The relative abundances of thermophilic bacteria had significant positive correlations with the abundance of sul1 in compost. The composting has a significant impact on the ARG-associated gut microbes in poultry manure. Variation partitioning analysis indicated that the change of bacterial community compositions and antibiotics contributed partially to the shift in ARG profiles. The results indicate that at industry-scale production broiler manure had more antibiotics and ARGs than layer manure did, and composting decreased most ARG abundances in poultry manure except for sulfonamide resistance genes.
相似文献The Yellow River Delta is the largest and youngest estuarine and coastal wetland in China and is experiencing the most active interactions of seawater and freshwater in the world. Bacteria played multifaceted influence on soil biogeochemical processes, and it was necessary to investigate the intermodulation between the soil factors and bacterial communities. Soil samples were collected at sites with different salinity degree, vegetations, and interference. The sequences of bacilli were tested using 16S rRNA sequencing method and operational taxonomic units were classified with 97% similarity. The soil was highly salinized and oligotrophic, and the wetland was nitrogen-restricted. Redundancy analysis suggested that factors related with seawater erosion were principal to drive the changes of soil bacterial communities and then the nutrient level and human disturbance. A broader implication was that, in the early succession stages of the coastal ecosystem, seawater erosion was the key driver of the variations of marine oligotrophic bacterial communities, while the increasing nutrient availability may enhance in the abundance of the riverine copiotrophs in the late stages. This study provided new insights on the characteristics of soil bacterial communities in estuarine and coastal wetlands.
相似文献