Biosequestration of lead using Bacillus strains isolated from seleniferous soils and sediments of Punjab

The present study was conducted to isolate and explore bacterial strains with a potential to sequester lead (Pb) and tolerate other heavy metals from industrial effluents and sediments. Out of the six bacterial strains isolated from seleniferous sites of Punjab, three isolates (RS-1, RS-2, and RS-3) were screened out for further growth-associated lead sequestration and molecular characterization on the basis of their tolerance toward lead and other heavy metals. Biomass and cell-free supernatant were analyzed for lead contents using ICP-MS after growth-associated lead sequestration studies in tryptone soya broth (pH?=?7.2?±?0.2) under aerobic conditions at 37 °C temperature. Almost 82 % and 70 % divalent lead was sequestered in cell pellets of RS-1 and RS-3, respectively while only 45 % of lead was found in cell pellet of RS-2 in the first 24 h. However, significant biosequestration of lead was observed in RS-2 after 48 h of incubation with concomitant increase in biomass. Simultaneously, morphological, biochemical, and physiological characterization of selected strains was carried out. 16S rRNA gene sequence of these isolates revealed their phylogenetic relationship with class Bacillaceae, a low G + C firmicutes showing 98 % homology with Bacillus sp.     

Can adaptive modulation of traits to urban environments facilitate Ricinus communis L. invasiveness?

This paper addresses the phenotypic variation among Ricinus communis L. populations in four urban habitat types (road verges, garbage dumps, construction debris, and natural area) in Delhi, India, by evaluating important traits such as plant height, basal circumference, seeds per plant, seed size, seed weight, specific leaf area, and reproductive index. An important biochemical marker, proline, considered as a good plant performance indicator under stress was also quantified in leaves of R. communis to evaluate its response in different habitats. Interestingly, the species showed significant variation in plant height, specific leaf area, seed size, seed weight, and leaf proline content in different habitat types. Leaf proline content was positively related to plant height, specific leaf area, and seed size while negatively related to the total number of seeds/plant. Interestingly, reproductive index, calculated as a ratio of the total number of seeds to the plant height also showed a negative relation with leaf proline content. Results indicated that R. communis exhibits adaptive modulation of growth, reproductive traits, and leaf proline content in various urban habitats which contributes to invasiveness, range expansion, and establishment of the species. The study also gives evidence of how morphological and physiological traits could directly affect invasiveness of R. communis.     

Can rare positive interactions become common when large carnivores consume livestock?

Livestock populations in protected areas are viewed negatively because of their interaction with native ungulates through direct competition for food resources. However, livestock and native prey can also interact indirectly through their shared predator. Indirect interactions between two prey species occur when one prey modifies either the functional or numerical responses of a shared predator. This interaction is often manifested as negative effects (apparent competition) on one or both prey species through increased predation risk. But indirect interactions can also yield positive effects on a focal prey if the shared predator modifies its functional response toward increased consumption of an abundant and higher-quality alternative prey. Such a phenomenon between two prey species is underappreciated and overlooked in nature. Positive indirect effects can be expected to occur in livestock-dominated wildlife reserves containing large carnivores. We searched for such positive effects in Acacia-Zizhypus forests of India's Gir sanctuary where livestock (Bubalus bubalis and Bos indicus) and a coexisting native prey (chital deer, Axis axis) are consumed by Asiatic lions (Panthera leo persica). Chital vigilance was higher in areas with low livestock density than in areas with high livestock density. This positive indirect effect occurred because lion predation rates on livestock were twice as great where livestock were abundant than where livestock density was low. Positive indirect interactions mediated by shared predators may be more common than generally thought with rather major consequences for ecological understanding and conservation. We encourage further studies to understand outcomes of indirect interactions on long-term predator-prey dynamics in livestock-dominated protected areas.     

Indoor–outdoor concentrations of RSPM in classroom of a naturally ventilated school building near an urban traffic roadway

A study on indoor–outdoor RSPM (PM₁₀, PM_2.5 and PM_1.0) mass concentration monitoring has been carried out at a classroom of a naturally ventilated school building located near an urban roadway in Delhi City. The monitoring has been planned for a year starting from August 2006 till August 2007, including weekdays (Monday, Wednesday and Friday) and weekends (Saturday and Sunday) from 8:0 a.m. to 2:0 p.m., in order to take into account hourly, daily, weekly, monthly and seasonal variations in pollutant concentrations. Meteorological parameters, including temperature, rH, pressure, wind speed and direction, and traffic parameters, including its type and volume has been monitored simultaneously to relate the concentrations of indoor–outdoor RSPM with them. Ventilation rate has also been estimated to find out its relation with indoor particulate concentrations. The results of the study indicates that RSPM concentrations in classroom exceeds the permissible limits during all monitoring hours of weekdays and weekends in all seasons that may cause potential health hazards to occupants, when exposed. I/O for all sizes of particulates are greater than 1, which implies that building envelop does not provide protection from outdoor pollutants. Further, a significant influence of meteorological parameters, ventilation rate and of traffic has been observed on I/O. Higher I/O for PM₁₀ is indicating the presence of its indoor sources in classroom and their indoor concentrations are strongly influenced by activities of occupants during weekdays.     

Green energy generation through PEHF – a blueprint of alternate energy harvesting

In this work, an attempt has been made to harvest green energy from piezoelectric material using fluid flow in a conduit. Piezoelectric Energy Harvesting using Fluid Flow (PEHF) experimental model has been designed and the outputs obtained are compared with results obtained from simulations using ANSYS (computational fluid dynamics) and also with the mathematical modeling. The PEHF model has been utilized to analyze the effect of flow rate of water with reference to energy extracted. The full wave bridge rectifier and voltage doubler circuits have been used to obtain the direct current (DC) from the PEHF model. It is observed that the output obtained using experiments holds good in agreement with the results retrieved through simulations and mathematical results. The increase in flow rate of fluid leads to initially increase and then decrease in output of PEHF model as the maximum energy generated when flow rates (external force) matches with the frequency of excitation of the systems, i.e., at its resonance. The maximum energy output is generated at its resonance frequencies. It is observed that the full wave bridge rectifier circuit gives greater output as compared to a voltage doubler circuit.     

Air assimilative capacity-based environment friendly siting of new industries--a case study of Kochi region, India

Air pollution has become a matter of grave concern, particularly in mega-cities and urban areas, where the situation is alarming and becoming more and more severe day-by-day and warrants, therefore, careful planning to facilitate future industrial development. Site selection, with the objective of minimizing adverse environmental impacts based on environmental criteria is a vital prerequisite, particularly for air polluting industries. In order to locate any air polluting industry, the assimilative capacity of the region needs to be assessed carefully and planned accordingly, so that the receiving environment is not adversely affected. Assimilative capacity of a region/airshed, widely represented through the ventilation coefficient by many researchers in the past, does not give a clear picture about the amount of emission load that can be assimilated in a given region. The ventilation coefficient, at best, can only present a broad picture about the air pollution dispersion potential (low, medium or high) of the region. A modified approach, which utilizes air quality modelling as a tool to estimate the maximum allowable emission load that a region can assimilate without violating the stipulated standards, has been used for estimating the assimilative capacity of the air environment. Details of this approach have been presented in this paper through a case study carried out for the Kochi region, located in the Kerala State of India. A variety of emission and meteorological scenarios have been considered and critical emission loads have been estimated. This approach shall provide necessary technical guidance to the environmental regulatory authorities as well as to the industries in planning environment friendly industrial development.     

Air pollution modeling for an industrial complex and model performance evaluation

Jamshedpur, the steel city of India situated in the eastern part of India is affected by increasing air pollution levels as a result of concentrated industrial activities. The impact of NOx emissions resulting from various air pollution sources, viz. industries, vehicles and domestic, was estimated using Industrial Source Complex Short-Term gaussian dispersion model. The contribution of NOx concentration from industrial, vehicular and domestic sources was found to be 53, 40 and 7%. Further statistical analysis was carried out to evaluate the model performance by comparing measured and predicted NOx concentrations. The model performance was found good with an accuracy of about 68%.     

Comparison of Two Manual Methods of Nitrogen Dioxide Determination in Ambient Air

Sodium arsenite (modified Jacobs and Hochheiser method; hereafter referred as SA), the most widely used manual monitoring method for determination of nitrogen dioxide (NO₂) in ambient air, particularly in developing countries has been evaluated and compared with US EPA recommended equivalent method of TGS-ANSA (hereafter referred as ANSA). NO₂ concentrations generated from laboratory test atmosphere were analyzed by these two methods and were compared statistically. Laboratory evaluations showed that SA method has high sensitivity to different sampling conditions, which normally vary during actual field monitoring. Hence, correction factors for absorption efficiency were estimated for SA and ANSA methods. Absorption efficiency of NO₂ in SA method was found to be much lower (64%) as against the reported value of 82% at the method recommended sampling conditions, whereas for ANSA method, it was found 1.0 as against the reported value of 0.93. After applying derived correction factors, both the methods produced almost similar concentration values of NO₂.     

Post-irrigation impact of domestic sewage effluent on composition of soils,crops and ground water--a case study

Long-term irrigation with sewage water adds large amounts of carbon, major and micro- nutrients to the soil. We compared the spatial distribution of N, P, K and other micronutrients and toxic elements in the top 0.6 m of an alluvial soil along with their associated effects on the composition of crops and ground waters after about three decades of irrigation with domestic sewage effluent as a function of distance from the disposal point. Use of sewage for irrigation in various proportions improved the organic matter to 1.24-1.78% and fertility status of soils especially down to a distance of 1 km along the disposal channel. Build up in total N was up to 2908 kg ha(-1), available P (58 kg ha(-1)), total P (2115 kg ha(-1)), available K (305 kg ha(-1)) and total K (4712 kg ha(-1)) in surface 0.15 m soil. Vertical distribution of these parameters also varied, with most accumulations occurring in surface 0.3 m. Traces of NO3-N (up to 2.8 mg l(-1)), Pb (up to 0.35 mg l(-1)) and Mn (up to 0.23 mg l(-1)) could also be observed in well waters near the disposal point thus indicating initiation of ground water contamination. However, the contents of heavy metals in crops sampled from the area were below the permissible critical levels. Though the study confirms that the domestic sewage can effectively increase water resource for irrigation but there is a need for continuous monitoring of the concentrations of potentially toxic elements in soil, plants and ground water.