Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts

Bisphenol A(BPA) has been shown to exert biological effects through estrogen receptor(ER)-dependent and ER-independent mechanisms. Recent studies suggest that prenatal exposure to BPA may increase the risk of childhood asthma. To investigate the underlying mechanisms in the actions of BPA, human fetal lung fibroblasts(h FLFs) were exposed to varying doses of BPA in culture for 24 hr. Effects of BPA on localization and uptake of BPA,cell viability, release of immune and developmental modulators, cellular localization and expression of ERα, ERβ and G-protein coupled estrogen receptor 30(GPR30), and effects of ERs antagonists on BPA-induced changes in endothelin-1(ET-1) release were examined.BPA at 0.01–100 μmol/L caused no changes in cell viability after 24 hr of exposure. h FLFs expresses all three ERs. BPA had no effects on either cellular distribution or protein expression of ERα, however, at 100 μmol/L(or 23 μmol/L intracellular BPA) increased ERβprotein levels in the cytoplasmic fractions and GPR 30 protein levels in the nuclear fractions.These paralleled with increased release of growth differentiation factor-15, decreased phosphorylation of nuclear factor kappa B p65 at serine 536, and decreased release of ET-1,interleukin-6, and interferon gamma-induced protein 10. ERs antagonists had no effects on BPA-induced decrease in ET-1 release. These data suggest that BPA at 100 μmol/L altered the release of immune and developmental modulators in h FLFs, which may negatively influence fetal lung development, maturation, and susceptibility to environmental stressors, although the role of BPA in childhood asthma remains to be confirmed in in vivo studies.     

Study of the effects of bisphenol A using human fetal lung fibroblasts

正Bisphenol A(BPA,2,2-bis(4-hydroxyphenyl)propane;CAS#80-05-7)is a highly produced industrial chemical,with an estimated four billion kilograms produced worldwide each year(reviewed in Vandenberg et al.,2010).BPA serves as a monomer in the manufacture of polycarbonate,a hard,clear plastic.Polycarbonate plastics are used in many consumer products,such as reusable water bottles.BPA is also widely used to make epoxy resins,which are used as coating on the inside of some     

Investigation of the blood biochemical status of gas station workers

The aim of this study was to examine occupation-associated effects on blood cytokine and immunoglobulin g (IgG) levels, antioxidant enzyme activities (SOD, GSHPx, and catalase), nitrite and nitrate levels, and malondialdehyde (MDA) concentrations in gas station workers (GSWs). Trace elements (Cu, Zn, and Pb) in serum, and the amount of hemoglobin in erythrocytes were also determined. Forty male GSWs and 30 male controls were included in the study. No significant difference was observed between the hemoglobin levels of GSWs and those in controls. Proinflammatory cytokine levels, such as tumor necrosis factor-α and interleukin-8 were significantly increased in GSWs’ sera. Interleukin-1β and interleukin-6 were below the detection limit of the assay in both the groups. Cytokine receptor interleukin 2R concentration and IgG levels were also significantly increased. Approximately, two-fold increase in nitrite and nitrate concentrations was found in GSWs’ sera. Erythrocyte antioxidant enzyme levels were significantly lower in GSWs’ blood. MDA concentration, a measure of lipid peroxidation, was found to be significantly elevated. Zn levels were significantly decreased, whereas Cu and Pb levels were increased in GSWs’ sera. Smoking was not found to be a major confounder for the association between exposure and blood parameters.