Relationships between anaerobic consortia and removal efficiencies in an UASB reactor degrading 2,4 dichlorophenol (DCP)

To gain more insight into the interactions between anaerobic bacteria and reactor performances (chemical oxygen demand-COD, 2,4 dichlorophenol-2,4 DCP removals, volatile fatty acid-VFA, and methane gas productions) and how they depended on operational conditions the microbial variations in the anaerobic granular sludge from an upflow anaerobic sludge blanket (UASB) reactor treating 2,4 DCP was studied. The study was composed of two parts. In the first part, the numbers of methanogens and acedogens in the anaerobic granular sludge were counted at different COD removal efficiencies. The relationships between the numbers of methanogens, the methane gas production and VFA production were investigated. The COD removal efficiencies increased to 74% from 30% while the number of total acedogens decreased to 10 from 30 cfu ml(-1). The number of total methanogens and acedogens varied between 11 x 10(3) and 10 x 10(9)MPN g(-1) and 10 and 30 cfu ml(-1) as the 2,4 DCP removal efficiencies were obtained between 60% and 99%, respectively. It was seen that, as the number of total acedogens decreased, the COD removal efficiencies increased. However, the number of total methanogens increased as the COD removal efficiencies increased. Correlations between the bacterial number and with the removal efficiencies obtained in different operational conditions were investigated. From the results presented in this paper a high correlation between the number of bacteria, COD removals, methane gas percentage, 2,4 DCP removals and VFA was observed. In the second part, methanogen bacteria in the anaerobic granular sludge were identified. Microbial observations and biochemical tests were applied to identify the anaerobic microorganisms from the anaerobic granular sludge. In the reactor treating 2,4 DCP, Methanobacterium bryantii, Methanobacterium formicicum, Methanobrevibacter smithii, Methanococcus voltae, Methanosarcina mazei, Methanosarcina acetivorans, Methanogenium bourgense and Methanospirillum hungatei were identified.     

Biotreatability and kinetics of UASB reactor to mixtures of chlorophenol pollutants

In most natural ecosystems heterotrophic microorganisms encountercomplex mixtures of carbon sources, each of which is present at aconcentration of few micrograms per litre. This study examined the biotreatability and kinetics of an upflow anaerobic sludge blanket (UASB) reactor to complex mixtures of chlorophenols encountered in environmental conditions using on-line and off-line experimental studies. Results indicate that (1) steady-state concentration was quite lower (98.3 mg L^-1) with complex mixture of chlorophenols than steady-state concentration achieved when only 2.4 dichlorophenol (124 mg L^-1) was studied alone on the same reactor; (2) that toxiceffects of chlorophenols increase with increasing concentrationsof toxicant. (Onset of the inhibitory effect occurred at a lowerconcentration in multi-substrate than in single substrate utilization); (3) addition of alternative utilizable substrate can mitigate toxic effects and enhance degradation; (4) the relative concentration of substrate was critical in determiningutilization patterns. HPLC analysis of off-line experimental samples resulted in a steady-state treatment efficiency of68% for COD, 36% for 2-hlorophenol, 40.5% for 4-chlorophenol, 70.7% for 2,4-dichlorophenol, 53.2% for 2,4,6-trichlorophenol and 42% for pentachlorophenol in presence of glucose. Kinetic constant in terms of V _max and K _s were determined. K _s for the five chlorophenols ranged between 0.016 and 0.117 kg m^-3 day^-1 while V _max ranged between 0.056 and 0.244 kg m^-3 day^-1.     

制药GMP技改工程水环境影响分析和对策

对湖北施恩堂制药一期GMP技术改造工程产生的水环境影响进行了分析,采用以UASB+SBR为主的工艺处理技改工程和原有工程产生的制药废水,"以新带老"削减,COD、BOD5、SS分别较GMP技改前减排94.6%、94.1%和50.2%,减轻了污染负荷,可实现达标排放,达到了增产减污的目的。     

UASB反应器中颗粒污泥的沉降性能与终端沉降速度

从流体力学角度,通过建立沉降速度模型探讨了UASB反应器中颗粒污泥的沉降性能与终端沉降速度．计算结果表明,(1)绝大多数颗粒污泥的沉降过程属于过渡区(1＜Re＜100)而非层流区,其沉降速度与直径成正比,可用Allen公式进行计算;(2)颗粒污泥的终端沉降速度远高于厌氧反应器中废水的上流速度,其良好的沉降性能解决了在高负荷情况下污泥的流失问题．所建模型能较好地反映实际条件下的情况．为厌氧反应器的工艺设计与正常运行提供理论依据．     

UASB系统中pH值与污泥浓度控制的探讨

介绍了啤酒生产废水处理工艺UASB系统,分析了pH值、污泥量对UASB系统的影响,并提出了保证出水水质达标的措施.     

UASB处理低浓度城市生活污水的中试试验

研究1·2m3厌氧反应器(UASB),在自然温度(水温32~5℃)下,处理CODCr浓度为200mg/L左右的低浓度生活污水。实验采用颗粒污泥接种二次启动法。水处理系统在水温32~5℃范围内运行6个月的时间,UASB反应器在上升流速为1·4~1·7m/h,水力停留时间(HRT)为3·0~2·5h,CODCr有机污泥负荷(SLR)为0·06~0·13kg/(kg·d)时,去除效率比较稳定,CODCr去除率达40%~80%。     

脱氮-混凝气浮-UASB-接触氧化法处理垃圾填埋渗滤液

采用脱氮—混凝气浮—UASB—接触氧化工艺处理高氨氮、高浊度的垃圾渗滤液，使处理后出水的各项指标完全达到国家《污水综合排放标准》(GB8978—1996)二级新扩改标准。对废水处理系统的工艺作了简单介绍，并对系统的调试、运行过程进行了技术总结。     

ABR反应器的设计

类似于多个UASB反应器串联起来运行的ABR反应器具有多种其它反应器不具备的优点，在处理化工颜料黄高盐生产废水的工程中，结合将高效复合微生物技术用于厌氧水解一连续曝气生化工艺取得成功；对ABR反应器而言，正确的设计参数是工程运行成功的保证。     

UASB反应器常温下处理生活污水的中试研究

中试试验研究讨论了利用颗粒污泥接种的UASB反应器常温下处理生活污水的运行性能。在7～31℃温度范围内处理生活污水,考察了不同温度、不同负荷条件下反应器的处理效率,讨论了各参数对反应器运行的影响,为UASB反应器常温下处理生活污水提供理论和实践指导。