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SO2衍生物对蚕豆根尖细胞不同分裂阶段的遗传损伤
引用本文:仪慧兰,司良燕,孟紫强. SO2衍生物对蚕豆根尖细胞不同分裂阶段的遗传损伤[J]. 应用与环境生物学报, 2002, 8(4): 383-386
作者姓名:仪慧兰  司良燕  孟紫强
作者单位:山西大学生命科学与技术学院环境医学与毒理学研究所,太原,030006
基金项目:山西省自然科学基金,山西省教委环境科学重点学科基金资助~~
摘    要:研究SO2体内衍生物-亚硫酸钠和亚硫酸氢钠混合液[c(Na2SO3):c(NaHSO3)=3:1]对蚕豆根尖细胞不同分裂时期的遗传损伤效应;结果表明:SO2衍生物(c=0.17-15.0mmolL^-1)诱发蚕豆根尖细胞遗传损伤在细胞分裂的不同阶段有不同的表现形式。间期异常主要是微核和核芽,分裂期异常包括微核,染色体断片,粘连及滞后等,研究结果表明:SO2衍生物处理组蚕豆根尖中,间期微核细胞数,分裂期具有染色体断裂,粘连或滞后的细胞数均明显多于对照组,而且易于观察分析,可以作为环境监测指标。

关 键 词:SO2衍生物 蚕豆根尖细胞 分裂阶段 遗传损伤 大气污染
修稿时间:2001-10-12

CYTOGENETIC DAMAGE AT MITOTIC STAGES OF VICIA FABA ROOT CELLS INDUCED BY SODIUM BISULFITE
YI Huilan,SI Liangyan , MENG Ziqiang. CYTOGENETIC DAMAGE AT MITOTIC STAGES OF VICIA FABA ROOT CELLS INDUCED BY SODIUM BISULFITE[J]. Chinese Journal of Applied and Environmental Biology, 2002, 8(4): 383-386
Authors:YI Huilan  SI Liangyan & MENG Ziqiang
Affiliation:YI Huilan,SI Liangyan & MENG Ziqiang **
Abstract:The cytogenetic damage induced in Vicia faba root tips by the derivatives of SO 2 -sodium bisulfite was studied. The results showed that the forms of cytogenetic damage at various division stages were different from each other. Most of the abnormal cells at interphase were micronucleus (MCN) and nuclear buds. The cells with one or more aberrations, such as MCN and chromosomal aberrations (CA) including chromosome segment, laggards and stickiness, were also obtained in split cells. The results also showed that the MCN frequencies at interphase and the frequencies of cells with CA at mitosis were significantly increased in the bisulfite groups, and more abnormal cells were easy to be observed. It was suggested that the derivatives of SO 2 were chromosome clastogen and V. faba root tips could be used to monitor SO 2 pollutant in the environment. Tab 6, Ref 13
Keywords:sodium bisulfite  sulfur dioxide  Vicia faba  cytogenetic damage
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