| 好氧反硝化菌种DF2的分离鉴定及生理生化特性分析 |
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| 引用本文： | 修海峰,朱仲元,丁爱中,郑蕾,赵轩.好氧反硝化菌种DF2的分离鉴定及生理生化特性分析[J].生态环境,2011(Z2). |
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| 作者姓名： | 修海峰 朱仲元 丁爱中 郑蕾 赵轩 |
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| 作者单位： | 内蒙古农业大学水利与土木建筑工程学院;北京师范大学水科学研究院//水沙科学教育部重点实验室; |
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| 基金项目： | 国家“十一五”科技支撑重大项目(2006BAJ04A072006BAJ08B01) |
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| 摘 要： | 分离了新型的高效反硝化细菌并研究其生物脱氮效率。通过观察形态学、生理生化鉴定和16SrRNA基因同源分析来鉴定菌种,致力于研究微生物污水脱氮处理的实际效果和克服反硝化过程中有害中间产物的积累。利用BTB培养基从水平潜流人工湿地基质中初步筛选出了9株平板阳性菌,革兰阴阳性均有。经试验研究发现该9种菌株在好氧条件下均具有一定的脱氮能力,其中以DF2和DF3 2种菌株脱氮能力最为显著,在3 d时间内NOX--N去除率达到了95%以上,NO2--N仅有微量积累;而其它7种菌株对NO3--N的去除率可以达到98%以上,但是NO2--N积累比较严重,积累量达到了NO3--N去除的50%~70%左右。经测序分析鉴定DF2和DF3分别属于德克斯氏菌属(Derxia)和假单胞菌属(Pseudomonadaceae)。德克斯氏菌属作为除氮的反硝化菌属在以往的研究中鲜有报道,该菌在好氧条件下可以合成周质NAR的亚基基因(napA)。
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| 关 键 词： | 好氧 反硝化菌属 筛选 基因 鉴定 |
Isolation and identification of the aerobic denitrifying strain DF2 and its physiological and biochemical analysis |
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| XIU Haifeng,ZHU Zhongyuan,DING Aizhong,ZHENG Lei,ZHAO Xuan .College of Hydraulic , Civil Engineering Inner Mongolia Agricultural University,Huhhot ,China,.College of Water Sciences,Beijing Normal University,Beijing.Isolation and identification of the aerobic denitrifying strain DF2 and its physiological and biochemical analysis[J].Ecology and Environmnet,2011(Z2). |
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| Authors: | XIU Haifeng ZHU Zhongyuan DING Aizhong ZHENG Lei ZHAO Xuan College of Hydraulic Civil Engineering Inner Mongolia Agricultural University Huhhot China College of Water Sciences Beijing Normal University Beijing |
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| Institution: | XIU Haifeng1,ZHU Zhongyuan1,DING Aizhong2,ZHENG Lei2,ZHAO Xuan2 1.College of Hydraulic and Civil Engineering Inner Mongolia Agricultural University,Huhhot 010018,China,2.College of Water Sciences,Beijing Normal University,Beijing 100875 |
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| Abstract: | The new and high efficiency denitrifying bacteria were isolated,and study on its biological nitrogen removal efficiency.Through the observation of morphology,physiological and biochemical identification and 16SrRNA gene homology analysis to identify bacteria,dedicated to the study actual effect of microbial nitrogen removal and overcomes accumulation of harmful intermediates in the denitrification process.Nine flat-positive bacteria are initially screened from horizontal subsurface flow constructed wetland ... |
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| Keywords: | aerobic denitrification bacteria genus screen gene identification |
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