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| 蓝藻发酵生产微生物农药的影响因素研究 | |
| 引用本文： | 蔡健,刘志,王跃强,徐荣险,周顺桂.蓝藻发酵生产微生物农药的影响因素研究[J].生态环境,2011,20(5):951-955. |
| 作者姓名： | 蔡健 刘志 王跃强 徐荣险 周顺桂 |
| 作者单位： | 1. 中牧实业股份有限公司,北京,100070 2. 湖南农业大学生物科学与技术学院,湖南长沙,410128 3. 广东省生态环境与土壤研究所,广东广州,510650 |
| 基金项目： | 国家农业科技成果转化资金项目(2010GB2E000347); 广东省科技成果转化启动项目(2010B060600010); 长沙市科技攻关项目(K0904037-11) |
| 摘 要： | 采用摇瓶发酵试验探讨了蓝藻为原料制备苏云金杆菌生物杀虫剂的可行性,并考察了不同培养条件（蓝藻含固率、种龄、接种量、初始pH、摇床转速、发酵温度）对苏云金杆菌生长增殖、产孢与产毒效果的影响。研究结果表明,无需任何预处理工序,Btk 130菌株能在蓝藻为唯一原料的培养基中正常生长发育,并且产孢产毒。发酵48 h后,芽孢产率达到86.7%,远高于常规培养基;生物毒效为282 IU.mL-1,与常规培养基相当。培养条件优化结果表明,在蓝藻含固率为2%、初始pH为7.0、接种物种龄为9 h、接种量为2%、培养温度为30℃、摇瓶转速为200 r.min-1的条件下培养48 h,Btk 130可达到较好的发酵效果,活菌数及抗热芽孢数可达7.32 CFU.mL-1和6.38 CFU.mL-1,生物毒效为528 IU.mL-1。该研究不仅为蓝藻提供了高附加值的处置新途径,而且可显著降低生物杀虫剂的生产成本,具有广阔的应用前景。 |
| 关 键 词： | 蓝藻 微生物农药 苏云金芽孢杆菌 培养条件 |
Factors affecting the production of Bacillus thuringiensis biopesticide using blue algae as raw material |
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| CUI Jian,LIU Zhi,WANG Yueqiang,XU Rongxian,ZHOU Shungui.Factors affecting the production of Bacillus thuringiensis biopesticide using blue algae as raw material[J].Ecology and Environmnet,2011,20(5):951-955. | |
| Authors: | CUI Jian LIU Zhi WANG Yueqiang XU Rongxian ZHOU Shungui |
| Institution: | CUI Jian 1,LIU Zhi 2,WANG Yueqiang3,XU Rongxian,ZHOU Shungui3 1.China Animal Husbandry Industry Co.Ltd.,Beijing 100070,China,2.College of Bioscience and Biotechology,Hunan Agricultural University,Changsha 410128,3.Guangdong Institute of Eco-Environmental and Soil Sciences,Guangzhou 510650 |
| Abstract: | This study investigated the bioconversion of blue algae into Bacillus thuringiensis based biopesticides.The shake-flask experiments were conducted to study the effects of solid concentration of blue algae,inoculum age,inoculation amount,initial pH,shake frequency and fermentation temperature on the bacterial growth,spore formation and toxin production by Bacillus thuringien-sis subsp.kurstaki(Btk).The experimental results showed that the blue algae wastewater,without any pretreatment procedures,was a potent... |
| Keywords: | blue-green algae biopesticide Bacillus thuringiensis fermentation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |