CFP(青色荧光蛋白)大肠杆菌用于表征好氧-厌氧反复耦合反应器细胞溶解特性的研究

构建了采用青色荧光蛋白(CFP)大肠杆菌快速示踪好氧-厌氧反复耦合(rCAA)反应器内溶胞特性的新方法.研究结果表明,rCAA反应器厌氧区域内的污泥上清液以及反应器后部好氧区域内的污泥上清液对大肠杆菌内CFP的释放具有明显的促进作用,说明这些区域的污泥上清液具有显著的溶菌作用.其中,厌氧区域污泥上清液对荧光细菌作用7h后的平均破碎率可以达到70%,且大肠杆菌胞内CFP的释放速度与初始荧光菌浓度以及污泥上清浓度有密切的关系.同时,研究结果进一步表明,厌氧区域污泥上清液具有的溶菌活性可能主要来自于污泥胞外的水解酶.

CFP as a biomarker for the tracing of the cell lysis process in an rCAA (repeated Coupling of Aerobes and Anaerobes) reactor

In order to characterize the process of the cellular destruction of microbes during the sludge reduction in an rCAA(repeated Coupling of Aerobes and Anaerobes)reactor,a novel method using CFP-harboring E.coli as the indicator was proposed.When the CFP cells were re-suspended in the sludge supernatant from the anaerobic compartments and the subsequent aerobic compartments in the rCAA reactor,the intracellular CFP was released from the bacterial cells,demonstrating that the sludge supernatant from these compa...