| Evaluation of Bacillus sp. MZS10 for decolorizing Azure B dye and its decolorization mechanism |
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| 作者姓名： | Huixing Li Ruijing Zhang Lei Tang Jianhua Zhang Zhonggui Mao |
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| 作者单位： | The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;School of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang 473004, China;The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China |
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| 基金项目： | This work was supported by the Science & Technology Program of Jiangsu Province (No. BE2011623) and the Scientific Research Project of Provincial Environmental Protection Bureau of Jiangsu Province (No. 2012047). |
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| 摘 要： | To evaluate decolorization and detoxification of Azure B dye by a newly isolated Bacillus sp. MZS10 strain, the cultivation medium and decolorization mechanism of the isolate were investigated. The decolorization was discovered to be dependent on cell density of the isolate and reached 93.55%(0.04 g/L) after 14 hr of cultivation in a 5 L stirred-tank fermenter at 2.0 g/L yeast extract and 6.0 g/L soluble starch and a small amount of mineral salts. The decolorization metabolites were identified with ultra performance liquid chromatography-tandem mass spectroscopy(UPLC-MS). A mechanism for decolorization of Azure B was proposed as follows: the C=N in Azure B was initially reduced to –NH by nicotinamide adenine dinucleotide phosphate(NADPH)-dependent quinone dehydrogenase, and then the –NH further combined with –OH derived from glucose to form a stable and colorless compound through a dehydration reaction. The phytotoxicity was evaluated for both Azure B and its related derivatives produced by Bacillus sp. MZS10 decolorization, indicating that the decolorization metabolites were less toxic than original dye. The decolorization efficiency and mechanism shown by Bacillus sp. MZS10 provided insight on its potential application for the bioremediation of the dye Azure B.
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| 关 键 词： | 脱色机理 芽孢杆菌 天青 染料 评价 烟酰胺腺嘌呤二核苷酸 超高效液相色谱 衍生产品 |
| 收稿时间： | 25 July 2013 |
| 修稿时间： | 9 October 2013 |
Evaluation of Bacillus sp. MZS10 for decolorizing Azure B dye and its decolorization mechanism |
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| Huixing Li,Ruijing Zhang,Lei Tang,Jianhua Zhang,Zhonggui Mao.Evaluation of Bacillus sp. MZS10 for decolorizing Azure B dye and its decolorization mechanism[J].Journal of Environmental Sciences,2014,26(5):1125-1134. |
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| Authors: | Huixing Li Ruijing Zhang Lei Tang Jianhua Zhang and Zhonggui Mao |
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| Institution: | The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;School of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang 473004, China;The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China |
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| Abstract: | To evaluate decolorization and detoxification of Azure B dye by a newly isolated Bacillus sp. MZS10 strain, the cultivation medium and decolorization mechanism of the isolate were investigated. The decolorization was discovered to be dependent on cell density of the isolate and reached 93.55% (0.04 g/L) after 14 hr of cultivation in a 5 L stirred-tank fermenter at 2.0 g/L yeast extract and 6.0 g/L soluble starch and a small amount of mineral salts. The decolorization metabolites were identified with ultra performance liquid chromatography-tandem mass spectroscopy (UPLC-MS). A mechanism for decolorization of Azure B was proposed as follows: the C=N in Azure B was initially reduced to -NH by nicotinamide adenine dinucleotide phosphate (NADPH)-dependent quinone dehydrogenase, and then the -NH further combined with -OH derived from glucose to form a stable and colorless compound through a dehydration reaction. The phytotoxicity was evaluated for both Azure B and its related derivatives produced by Bacillus sp. MZS10 decolorization, indicating that the decolorization metabolites were less toxic than original dye. The decolorization efficiency and mechanism shown by Bacillus sp. MZS10 provided insight on its potential application for the bioremediation of the dye Azure B. |
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| Keywords: | Azure B Bacillus sp decolorization ultra performance liquid chromato graphy-tandem mass spectroscopy phytotoxicity |
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