| 产中性纤维素酶芽孢杆菌Y106产酶条件优化 |
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| 引用本文： | 陈士成,曲音波,张岩,张颖,高培基.产中性纤维素酶芽孢杆菌Y106产酶条件优化[J].应用与环境生物学报,2000,6(5):457-461. |
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| 作者姓名： | 陈士成 曲音波 张岩 张颖 高培基 |
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| 作者单位： | 山东大学微生物技术国家重点实验室济南 250100 |
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| 基金项目： | 国家“九五”科技攻关项目 |
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| 摘 要： | 筛选得到一株高产中性纤维素酶的芽孢杆菌Ｙ１０６，并对其发酵条件进行了优化，麸皮能够较大幅度地促进产酶，果糖是良好的诱导物，有机氮流促进产酶的效果优于无机氮源，最优的有机氮源是蛋白胨。Ｆｅ＾３＋、Ｆｅ＾２＋、Ｎａ＾＋、Ｃａ＾２＋可以促进纤维素酶的合成，而Ｃｕ＾２＋、Ａｇ＾＋、Ｃｏ＾２＋、Ｈｇ＾２＋则对合成起遏制作用，在酶反应系统中Ｆｅ＾３＋、Ｆｅ＾２＋、Ｃａ＾２＋、Ｍｇ＾２＋能激活纤维素酶活性，Ｃｏ
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| 关 键 词： | 芽孢杆菌 中性纤维素酶 响应面分析 产酶 |
| 修稿时间： | 1999年12月17 |
OPTIZATION OF CONDITIONS FOR SUBMERGED FERMENTATION OF NEUTRAL C ELLULASE BY BACILLUS SP. Y106 |
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| CHEN Shiche ng,QU Yinbo,ZHANG Yan,ZHANG Ying,GAO Peiji.OPTIZATION OF CONDITIONS FOR SUBMERGED FERMENTATION OF NEUTRAL C ELLULASE BY BACILLUS SP. Y106[J].Chinese Journal of Applied and Environmental Biology,2000,6(5):457-461. |
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| Authors: | CHEN Shiche ng QU Yinbo ZHANG Yan ZHANG Ying GAO Peiji |
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| Abstract: | A strain Y106 nighly produring extracellular neutral cellulase was screened and identified as Bacillus sp. The fermentation conditions in shaking flasks were investigated. The highest lever of cellulase activity was induced in the medium containing wheat bran. Fructose was a excellent inducer. The efficiency of organic nitrogen sources was better than that of inorganic nitrogen sources, while peptone was the best organic nitrogen source. Cellulase production could be improved by Fe 2 , Fe 3 , Na , and Ca 2 , while inhabited by Cu 2 , Ag , Co 2 , and Hg 2 . The components of the medium were optimized by the method of RSA(Response Surface Analysis). When Y106 was cultured under the optimum conditions, cellulase production could reach 4.57 IU mL -1 . Fig 3, Tab 6, Ref 7 |
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| Keywords: | Bacillus neutral cellulase RSA (Response Surface Analysis) |
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