| 油松叶绿体DNA间隔序列特点及系统学意义 |
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| 引用本文： | 李毳,柴宝峰.油松叶绿体DNA间隔序列特点及系统学意义[J].应用与环境生物学报,2010(2). |
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| 作者姓名： | 李毳 柴宝峰 |
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| 作者单位： | 山西财经大学环境经济学院;山西大学生物技术研究所; |
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| 基金项目： | 山西省回国留学人员科研项目(No.200846); 国家自然科学基金项目(No.30770294)资助~~ |
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| 摘 要： | 对全国分布区12个天然油松种群进行取样,测定了叶绿体DNAtrnT-trnL、trnS-trnG、trnL-trnF基因间隔序列,分析了序列碱基组成特点及在松科系统分类中的意义.测序结果显示,3个片段碱基序列均富含A/T,长度依次为448bp、636bp和421bp,所测片段在种群水平上十分保守,没有发现具有种内鉴别意义的碱基变异.但此片段适合于松科属间和种间的系统学分析,运用PHYLIP软件构建松科植物的邻接系统树,支持松科分为两个亚科的分类系统,拓扑结构表明松属与银杉属的关系最近.由于叶绿体DNA序列长度适中,扩增和测序较为容易,适合作为研究松科植物系统进化较为理想的分子标记.图2表2参24
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| 关 键 词： | 油松 cpDNA间隔序列 系统进化 分子标记 |
Characteristics and Systematic Significance of Chloroplast DNA Sequences of Pinus tabulaeformis |
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| LI Cui & CHAI Baofeng.Characteristics and Systematic Significance of Chloroplast DNA Sequences of Pinus tabulaeformis[J].Chinese Journal of Applied and Environmental Biology,2010(2). |
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| Authors: | LI Cui & CHAI Baofeng |
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| Institution: | LI Cui1 & CHAI Baofeng2 (1Faculty of Environment Economics,Shanxi University of Finance , Economic,Taiyuan 030006,China) (2Institute of Biotechnology,Shanxi University,China) |
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| Abstract: | The samples of Pinus tabulaeformis were collected from 12 populations occurring in whole China within its chloroplast DNA distribution range, and their noncoding sequences of chloroplast DNA trnT-trnL, trnS-trnG and trnL-trnF were sequenced. The characteristics of base compositions and the significance of the sequences in taxonomy of Pinaceae were analyzed by using the sequence data. The results indicated: (1) The three cpDNA sequences, 448 bp, 636 bp and 421 bp length, were characterized with a high A+T co... |
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| Keywords: | Pinus tabulaeformis cpDNA spacer sequence systematic evolution molecular marker |
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