由一株青霉菌产生的聚乙烯醇降解酶 |
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引用本文: | 钱鼎,李寅,堵国成,陈坚.由一株青霉菌产生的聚乙烯醇降解酶[J].应用与环境生物学报,2004,10(2):226-230. |
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作者姓名: | 钱鼎 李寅 堵国成 陈坚 |
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作者单位: | 1. 江南大学工业,生物技术教育部重点实验室,江苏,无锡,214036;江南大学工业,生物工程学院,江苏,无锡,214036 2. 江南大学工业,生物工程学院,江苏,无锡,214036 |
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基金项目: | 国家高技术研究发展计划(863)项目(科技部)资助(No.2003AA322051)~~ |
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摘 要: | 从纺织污水活性污泥中筛选得到一株新型聚乙烯醇(PVA)降解酶产生菌,根据形态学特征鉴定该菌属于青霉属(Penicillium sp.),实验室编号WStt02-21.这是由霉菌产生PVA降解酶的首例报道.在考察了菌株WSH02-21基本生长和产酶特性的基础上,研究了营养条件对PVA降解酶合成的影响.通过对营养条件的单因素考察和正交试验,确定了最优培养条件为PVA 40 g L-1、葡萄糖3.0 g L-1、NH4Cl 8.0 g L-1、KH2PO4 2.0 g L-1、酵母膏1.0 g L-1、:MgSO40.5 g L-1。、CaCl2 1.0 g L-1、NaCl 0.02 g L-1、FeSO4·7H2O 0.02 g L-1,初始pH 6.4.其中PVA浓度是影响Penicilliumsp.WSH02-21合成PVA降解酶的最重要因素.采用最优化条件进行验证试验,PVA降解酶酶活(4.4 U mL-1)略高于正交试验中的的最高酶活(4.3 U mL-1).图7表2参11
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关 键 词: | 青霉菌 聚乙烯醇降解酶 聚乙烯醇 PVA 工业废水 |
修稿时间: | 2003年7月14日 |
EFFECT OF CULTURE CONDITIONS ON PRODUCTION OF POLYVINYL ALCOHOL-DEGRADING ENZYME |
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Abstract: | A new strain that produced polyvinyl alcohol - degrading enzyme was isolated from activated sludge of a textile factory. Morphological characteristics show that this strain belongs to Penicillium sp. As we know, it was first reported that PVA-degrading enzyme was produced by a strain of fungi. The effect of culture conditions on the production of polyvinyl alcohol - degrading enzyme was also studied. By single-factor and orthogonal experiment methods, the optimal conditions were determined as:PVA40 gL-1, glucose 3.0 g L-1, NH4Cl 8. 0 g L-1, KH2PO4 2.0 g L-1, Yeast-extract 1.0 g L-1, MgSO40. 5 g L-1, CaCl2 1. 0 g L-1, NaCl 0. 02 g L-1, FeSO4 ?7H2 O 0. 02 g L-1 and initial pH 6. 4. PVA was proven to be the most important factor that affected the production of PVA-degrading enzyme. By applying the optimal conditions, the PVA-degrading enzyme activity was 4.4 U mL-1 , which was slightly higher than the best result from orthogonal experiment (4. 3 U mL-1). Fig 7, Tab 2, Ref 11 |
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Keywords: | polyvinyl alcohol PVA-degrading enzyme Penicillium sp orthogonal experiment |
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