| 螺旋藻多糖的提取分离纯化及结构初步研究 |
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| 引用本文： | 李亚清,杨海波,刘艳,张淑芬. 螺旋藻多糖的提取分离纯化及结构初步研究[J]. 海洋环境科学, 2004, 23(2): 75-77 |
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| 作者姓名： | 李亚清 杨海波 刘艳 张淑芬 |
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| 作者单位： | 1. 大连理工大学,精细化工国家重点实验室,辽宁,大连,116012
2. 大连大学,化学与化学工程系,辽宁,大连,116622 |
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| 基金项目： | 辽宁省博士科研启动基金资助项目(001059) |
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| 摘 要： | 离心收集处于生长平衡后期的螺旋藻(Spirulinamaxima),通过渗透冲击协同冻融细胞破碎、冷水提取,TCA除蛋白、乙醇沉淀得到粗多糖。用SephadexG 50凝胶层析分离纯化,得到一种水溶性螺旋藻多糖。利用醋酸纤维薄膜电泳和SephadexG 100凝胶检验螺旋藻多糖纯度,证明该糖组分均一。根据红外光谱,元素分析的结果对该糖结构进行初步研究。
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| 关 键 词： | 螺旋藻多糖 分离纯化 结构分析 |
| 文章编号： | 1007-6336(2004)02-0075-03 |
| 修稿时间： | 2003-10-30 |
Studied on the extraction and purification and structure of Spirulina maxima |
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| LI Yaqing,YANG Hai-bo,LIU Yan,ZHANG Shu-fen. Studied on the extraction and purification and structure of Spirulina maxima[J]. Marine Environmental Science, 2004, 23(2): 75-77 |
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| Authors: | LI Yaqing YANG Hai-bo LIU Yan ZHANG Shu-fen |
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| Affiliation: | LI Yaqing~1,YANG Hai-bo~2,LIU Yan~2,ZHANG Shu-fen~1 |
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| Abstract: | Centrifuging and harvesting Spirulina maxima at the stationary phase, and fragmentizing cells by penetrated and frozen-melt, then extracted by cold water, removing proteins by TCA, at last, the raw polysaccharide was deposited by ethanol. A kind of water-dissolved Spirulina maxima polysaccharide was obtained by SephadexG-50 gel chromatography. The composition of this polysaccharide was uniformed by means of cellulose film electrophoresis and SephadexG-100 gel chromatography. The structure of this polysaccharide was the primarily study by means of IR analysis and element analysis. |
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| Keywords: | Spirulina maxima polysaccharide separation purification structure-analysis |
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