黄单胞菌生物滴滤塔处理硫化氢能力及产物研究

以黄单胞菌为硫氧化菌种挂膜于生物滴滤塔,研究其去除能力及产物.通过测定压降、生物量确定挂膜时间,以进出气口浓度变化反映去除效率,测定营养液pH值变化,用SEM电镜扫描、能谱分析、X射线衍射对产物进行分析.结果表明,黄单胞菌挂膜时间为4 d,持续运行,菌种均匀地分布于填料表面;保持气体流量为1 L/min,H_2S气体质量浓度在2 000 mg/m~3以下时,去除率大于90.5%,单位体积最大生化去除量为43.23 g/(m~3·h);以10 d为周期添加营养,系统的pH值在5～7;自然沉降得到的黄色颗粒状沉淀粒径为3.161～31.90 μm;能谱分析表面主要成分为C、O、S,应该为微生物和S系物;X射线衍射分析表明干燥粉末的主要无机物质存在形态为S单质.塔在弱酸环境运行,能减少对装置的腐蚀,营养液中通过物理沉降、过滤、低速离心的简单手段,就能回收产物S单质.

Hydrogen-sulfide removing capability and the product of bio-trickling to be filtered with Xanthomonas sp.

The purpose of the present paper is to introduce our study results of the hydrogen-sulfide removing power by using the xanthomonas sp. as sulfur oxidation bacteria in a continuous glass column bioreactor with a diameter of 90 mm and an effective packing height of 200 mm. As is known, hydrogen sulfide (H_2S) is an extremely high toxic odor that is produced naturally during the natural cyclic movement and harmful to human activities as well as to the other living organisms. It is just for its detrimental nature that it is ruled as one of the most serious items in the emission control regulation known as GB 14554-93 in China. In order to find out the film-forming characteristics, we have done investigations with the starting time of Xanthomona sp. by using its pressure drop and biofilm biomass. In proceeding with our research, we have done regular removing simulation experiments, measured the continuous operation, and pH changes of its nutrient solution. Experiments show that the uniform distribution of Xanthomonas sp. on the filler surface in 4d time is about 1.2-1.3 mg/g. At the H_2S gas concentrations of the following 2 000 mg/m~3, while maintaining 1 L/min flowrate, the removing rate can be made higher than 90.5% with EC 43.23 g/(m~3·h), After that, in the following 5 156 mg/m~3, on the condition of maintaining 1 L/min flowrate, the removing rate can also be made higher than 53.2% with EC being 59.73 g/(m~3·h). Keeping well-fed, no downward trend can be found with the pH values between 57 in every other 10 days. Under the weak acid conditions, corrosion of the trickling filter and pipeline can be found a bit reduced with yellowish granular particle subsidences left over in the nutrient solution. Whereas the granular particle size is usually 3.161-31.90 μm, the principal components are C, 11.42%, O, 18.19%, S, 15.85%, respectively, which can be seen by the surface energy spectrum analysis. In addition, X-ray diffraction analysis of the main dry powder form of material existence, the granular particle is found of single-S quality. Thus, by means of natural subsidence, filtering, low-speed centrifugation, the product of S can be made recycling easily. In addition, Xanthomonas sp. proves to be feasible be used in bio-trickling reactor to remove H_2S in the weak acid condition with the product of single-S quality.