异养硝化-好氧反硝化菌的筛选及脱氮性能的实验研究

通过极限稀释和显色培养基相结合筛选的方法,从畜禽养殖废水样品中筛选到1株同时具有异养硝化-好氧反硝化双重功能的细菌CPZ24.该菌株革兰氏染色呈阳性,杆状,菌落颜色为橙红色.经形态、生理生化特性,16S rDNA序列分析,初步鉴定该菌为嗜吡啶红球菌(Rhodococuus pyridinivorans).对该菌进行异养硝化功能和好氧反硝化功能进行研究,结果表明,在异养硝化过程中,该菌可将培养基中的氨氮全部去除,其中对总氮的去除率可达98.70%;在好氧反硝化过程中,该菌对硝酸盐氮的去除率可达到66.74%,总氮的去除率达到64.27%.此高效脱氮降解菌可实现自身同步硝化-反硝化脱氮功能,能够独立完成生物脱氮的全过程.

Screening and Denitrification Characteristics of a Heterotrophic Nitrification-Aerobic Denitrifier Bacteria

A heterotrophic nitrification-aerobic denitrifier bacteria CPZ24 was isolated form the livestock wastewater by way of the limiting dilution combined with the chromogenic medium screening methods. This bacterium was Gram positive, rod. The colonies of the strain were orange-red.It was identified as Rhodococuus pyridinivorans according to its morphological and physiological properties and the analysis of its 16S rDNA gene. Studied on its function of heterotrophic nitrification and aerobic denitrification,the results show that all NH⁺₄-N is removed and the removal rate of TN is 98.70% in heterotrophic nitrification; the removal rate of NO^-₃-N by this strain is 66.74% and the removal rate of TN is 64.27%.This high effective microorganisms with nitrogen removed is able to realize simultaneous nitrification and denitrification.It can perform the whole process of bacteria denitrification independently.