餐厨垃圾发酵液强化潮汐流人工湿地对污水厂尾水脱氮效能

采用餐厨垃圾发酵液(food waste fermentation liquid, FWFL)作为潮汐流人工湿地(tidal flow constructed wetland, TFCW)外加碳源，考察其对污水处理厂尾水湿地脱氮效果的影响，并通过湿地氮转化速率、酶活性测定及微生物群落结构分析探究其机理。结果表明：投加FWFL后人工湿地中TN、NO₃^--N、TP的去除率分别提高了15.7%~36.2%、3.3%~42.3%、11.2%~45.8%，且FWFL的添加不会对出水NH₄⁺-N和COD产生显著影响；FWFL可改善TFCW低温时的脱氮效果；投加FWFL后TFCW的反硝化速率、反硝化酶活性以及电子传递系统活性均有所提高，TFCW微生物的丰富度和多样性明显提高，微生物群落结构也趋于稳定，反硝化菌群大量增加。变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)与念珠菌门(Candidatus Saccharribacteria)为优势菌门，水杆菌属(Aquabacterium)与产丁酸盐细菌属(Saccharibacteria genera incertae sedis)为优势菌属。

Enhanced denitrification of sewage treatment plant effluent by tidal flow constructed wetland with food waste fermentation liquid addition

The food waste fermentation liquid (FWFL) was used as the carbon source of the tidal flow constructed wetland (TFCW), and the effect of FWFL addition on nitrogen removal of sewage treatment plant effluent was investigated. Furthermore, the denitrification mechanism was explored through the determination of wetland nitrogen conversion rate, enzyme activity and microbial community structure. The results showed that, after adding FWFL in the TFCW, the removal rates of TN, NO3−-N and TP in sewage treatment plant effluent increased by 15.7%~36.2%, 3.3%~42.3% and 11.2%~45.8%, respectively, and the addition of FWFL did not have a significant impact on effluent NH4+-N and COD, while it could improve the denitrification effect of the TFCW at low temperatures; the rates of denitrification, denitrification enzyme activity and electron transport system activity of the TFCW increased with FWFL addition; the richness and diversity of wetland microorganisms were significantly improved, the microbial community structure also tended to be stable, and the denitrifying microbiota increased significantly. Proteobacteria, Bacteroidetes, Candidatus Saccharribacteria were the dominant phylums, Aquabacterium and Saccharibacteria genera incertae sedis were the dominant genus.