邻苯二甲酸二乙基己酯致OVA致敏大鼠哮喘样病理学改变

全球哮喘患者人数众多,而且这一数字仍在不断增长.近年来大家把目光聚焦到人造化学物质与哮喘增加的关系上.邻苯二甲酸二乙基己酯(di-(2-ethylhexyl)phthalate,DEHP)是现代社会一种常见的污染物,在室内环境中也有广泛的存在.本研究通过建立卵清白蛋白(OVA)致敏的大鼠哮喘模型,来探讨DEHP对哮喘的影响.将Wistar大鼠随机分为6组,分别为生理盐水对照和两种浓度(0.5mg·m-3和5mg·m-3)的DEHP染毒的无OVA致敏组,以及这三者对应的OVA致敏组.行肺部滴注法对大鼠进行染毒,每天1次,染毒剂量相当于在0.5mg·m-3和5mg·m-3浓度的DEHP中暴露8h.处理后的大鼠通过肺功能检测,ELISA检测肺组织细胞因子和肺组织切片等方法研究其哮喘症状.结果显示DEHP染毒的OVA致敏组具有更显著的哮喘症状.

Effect of di-(2-ethylhexyl) phthalate on asthmatic pathologic changes in ovalbumin-immunized rats

Around the world, many people suffer from asthma, and the number of cases keeps growing. Recently, many researchers are focusing on the relationship between man-made chemicals and the growing incidence of asthma. Di-(2-ethylhexyl) phthalate (DEHP) is a common pollutant in modern life, and it is easily found in indoor environments. We explored the relationship between DEHP and asthma by using a rat asthma model. Six groups of rats were used, which are control, two DEHP exposure groups (0.5 mg·m-3 and 5 mg·m-3) with non OVA rats and another 3 groups of OVA-induced rats with the same exposures. Lung function, cytokines and histological examination of lungs were used to estimate asthmatic changes in these rats. The DEHP exposure groups showed more severe asthmatic pathological changes.