| 两株自养型氨氧化细菌的分离、鉴定及系统发育分析 |
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| 引用本文： | 窦立军,蒋进元,黄永亨,周岳溪,崔俊涛,李 越.两株自养型氨氧化细菌的分离、鉴定及系统发育分析[J].环境科学研究,2011,24(11):1312-1317. |
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| 作者姓名： | 窦立军 蒋进元 黄永亨 周岳溪 崔俊涛 李 越 |
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| 作者单位： | 1.吉林农业大学资源与环境学院, 吉林 长春 130118;中国环境科学研究院水污染控制技术研究中心, 北京 100012 |
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| 基金项目： | 国家高技术研究发展计划(863)项目(2008AA10Z406) |
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| 摘 要： | 以水产养殖废水处理工程的生物膜为菌源，采用传统的富集、分离纯化等微生物学手段，筛选出2株自养型氨氧化细菌A.P-7和A.P-8，并将其转接至液体分离培养基中培养17 d，定量检测结果表明，菌株A.P-7和A.P-8的氨氮去除率均可达到90%以上. 对2株菌的形态特征与生理生化性质分析结果表明,2株菌与假单胞菌属成员相似. 进一步测定2株菌的16S rDNA基因序列，测序结果提交至GenBank进行同源性检索分析，并通过MEGA 4.0软件进行比对和系统发育分析，结果显示2株菌均属于假单胞菌属，与门多萨假单胞菌的同源性最高，达99%以上.
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| 关 键 词： | 氨氧化细菌 富集 分离 克隆 系统发育 |
| 收稿时间： | 2011/1/26 0:00:00 |
| 修稿时间： | 2011/5/4 0:00:00 |
Separation, Identification and Phylogenetic Analysis of Two Strains of Autotrophic Ammonia Oxidizing Bacteria |
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| DOU Li-jun,JIANG Jin-yuan,HUANG Yong-heng,ZHOU Yue-xi,CUI Jun-tao and LI Yue.Separation, Identification and Phylogenetic Analysis of Two Strains of Autotrophic Ammonia Oxidizing Bacteria[J].Research of Environmental Sciences,2011,24(11):1312-1317. |
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| Authors: | DOU Li-jun JIANG Jin-yuan HUANG Yong-heng ZHOU Yue-xi CUI Jun-tao and LI Yue |
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| Institution: | DOU Li-jun1,2,JIANG Jin-yuan2,HUANG Yong-heng3,ZHOU Yue-xi2,CUI Jun-tao1,LI Yue1 1.College of Resources and Environment,Jilin Agricultural University,Changchun 130118,China 2.Research Center of Water Pollution Control Technology,Chinese Research Academy of Environmental Sciences,Beijing 100012,China 3.Bureau of Aquatic Products and Fisheries Administration in Weiyuan County,Weiyuan 642450,China |
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| Abstract: | Two strains of autotrophic Ammonia-Oxidizing Bacteria(AOB) A.P-7 and A.P-8 were isolated from the biofilm of an aquaculture wastewater treatment plant using traditional microbiology means including enrichment,isolation and purification,transferred to liquid separation medium and cultured for 17 days.Quantitative test results indicated that the ammonia nitrogen conversation rate of the two strains A.P-7 and A.P-8 could both reach above 90%.The morphological characteristics and physiological and biochemical p... |
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| Keywords: | ammonia oxidizing bacteria enrichment separation cloning phylogenetic analysis |
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