| 一株好氧反硝化菌的鉴定及反硝化特性研究 |
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| 引用本文： | 许尚营,张华,李娜,李风,杨俊忠,郑永良,刘德立.一株好氧反硝化菌的鉴定及反硝化特性研究[J].环境科学与技术,2010,33(2). |
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| 作者姓名： | 许尚营 张华 李娜 李风 杨俊忠 郑永良 刘德立 |
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| 作者单位： | 1. 华中师范大学生命科学学院,湖北省遗传调控与整合生物学重点实验室,湖北,武汉,430079
2. 华中师范大学生命科学学院,湖北省遗传调控与整合生物学重点实验室,湖北,武汉,430079;黄冈师范学院生命科学与工程学院,湖北,黄冈,438000 |
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| 基金项目： | 教育部博士点基金项目(20060511002);;“211”重点学科建设项目;;湖北省科技攻关计划项目(2007AA201C50,2007AA301C26);;湖北省教育厅优秀中青年人才项目(Q200727005) |
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| 摘 要： | 从湖北某纺织厂废水生化处理池中分离得到1株好氧反硝化细菌HS-N2,并对其反硝化能力进行了研究。结果表明,HS-N2在能有效去除培养基中的硝酸盐氮。在好氧条件下,30℃培养24h,能将10mmol/L硝酸盐氮去除95%以上;在兼性厌氧条件下,24h的脱氮率达到85%。研究了该菌株的好氧反硝化特性。结果表明:其反硝化最适温度和pH分别为30℃和7.0。设计特异性引物,扩增HS-N2菌株的16SrDNA并测序,运用Blast比对构建了进化树,发现该菌与肠炎沙门氏菌(Salmonella enteritidis)的同源性高达100%。结合形态观察和生理生化鉴定,初步鉴定该菌属于沙门氏菌属(Salmonella),命名为Salmonella sp.HS-N2。目前关于沙门氏菌属具有反硝化能力的菌株鲜有报道。
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| 关 键 词： | 好氧反硝化 沙门氏菌属 系统发育 |
Isolation of a New Aerobic Denitrifying Bacterium and Its Denitrification Characteristics |
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| XU Shang-ying,ZHANG Hua,LI Na,LI Feng,YANG Jun-zhong,ZHENG Yong-liang,LIU De-li.Isolation of a New Aerobic Denitrifying Bacterium and Its Denitrification Characteristics[J].Environmental Science and Technology,2010,33(2). |
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| Authors: | XU Shang-ying ZHANG Hua LI Na LI Feng YANG Jun-zhong ZHENG Yong-liang LIU De-li |
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| Institution: | 1.Laboratory of Genetic Regulation and Integrative Biology;School of Life Science;Central China Normal University;Wuhan 430079;China;2.School of Life Science and Engineering;Huanggang Normal University;Huanggang 438000;China |
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| Abstract: | A bacterial strain HS-N2 was isolated from a textile mill in Hubei Province. Results showed that HS-N2 was capable of denitrification under aerobic condition and facultative anaerobic condition. Nitrate of 10mmol/L could be effi-ciently removed by strain HS-N2 and the nitrogen removal rate was above 95% at 30℃ for 24h culture. Factors'that af-fecting anaerobic denitrification by swain HS-N2 were determined. Results indicated that the most optimum temperature and pH value for aerobic denitrification were at 30℃ and 7.0 respectively. According to morphological characteristics, physiological biochemical test and sequence analysis of 16S rDNA, strain HS-N2 was identified as Salmonella Lignieres. |
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| Keywords: | aerobic denitrification Salmonella Lignieres phylogenetic analysis |
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