24孔板发酵法测定地表水和废水中粪大肠菌群的研究

对地表水和废水中粪大肠菌群的多管发酵法进行了改进。改进了初发酵方法,采用24孔板代替试管进行初发酵,复发酵方法保持不变。对环境中采集的23个污水样本采用24孔板初发酵法与多管发酵法进行比较,结果表明,两种方法相关性良好,两者在结果上无统计学意义的差别。采用改进的24孔板法进行初发酵有效节约了培养基、缩短了实验准备时间,简化了操作步骤,尤其适用于大批量的、污染严重的地表水和废水中粪大肠菌群的检测。

Research on Determination of Fecal Coliform in Surface Water and Wastewater with 24-well Plates Fermentation

Multiple tube fermentation methods for fecal coliform in surface water and wastewater has been improved in this study. It improved the preliminary fermentation method using 24-well plates instead of test tubes,and the re-fermentation methods remain unchanged. Then,the 24-well plates fermentation method was compared with multiple tube fermentation method for 23 sewage samples collected in the environment. The results showed a good correlation between the two methods,the results of two methods have no significant difference. The improved method can effectively save the medium and shorten test preparation time,simplifies the procedure,especially for large quantities of polluted surface water and wastewater fecal coliform detection.