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北仑河口红树林沉积物微生物在潮间带的分布状况
引用本文:熊小飞,王欣,周浩郎,吴鹏.北仑河口红树林沉积物微生物在潮间带的分布状况[J].海洋环境科学,2018,37(1):101-106.
作者姓名:熊小飞  王欣  周浩郎  吴鹏
作者单位:1.国家海洋局南海环境监测中心, 广东 广州 510300
基金项目:国家海洋局近岸海域生态环境重点实验室基金资助项目(201608);广西红树林保护与利用重点实验室开放基金课题(GKLMC-201501);广东省自然科学基金项目(2014A030310495);国家海洋局南海分局海洋科学技术局长基金资助项目(1516)
摘    要:以16S rRNA基因为分子标记,通过高通量测序方法分析了广西北仑河口高、中和低3个潮间带中红树林沉积物微生物群落结构。结果表明:北仑河口潮间带红树林沉积物中微生物多样性丰富,微生物Shannon指数变化范围在10.2~10.4之间。潮间带红树林微生物优势类群为Proteobacteria,Chloroflexi,Bacteroidetes,Planctomycetes和Acidobacteria。OTUs的维恩图表明3个潮间带中红树林微生物组成相似性较高。然而,热图和三相图分析发现潮间带的微生物种属特异性具有差异。高通量测序还发现北仑河口红树林中存在一些微生物参与有机物污染物降解和碳氮等元素循环。

关 键 词:红树林    潮间带    沉积物    微生物群落    高通量测序
收稿时间:2017-05-06

The distribution of mangrove bacteria in intertidal surface sediments of the Beilun estuary
Xiao-fei XIONG,Xin WANG,Hao-lang ZHOU,Peng WU.The distribution of mangrove bacteria in intertidal surface sediments of the Beilun estuary[J].Marine Environmental Science,2018,37(1):101-106.
Authors:Xiao-fei XIONG  Xin WANG  Hao-lang ZHOU  Peng WU
Institution:1.South China Sea Environmental Monitoring Center, State Oceanic Administration, Guangzhou 510300, China
Abstract:The mangrove bacterial composition in three intertidal zones (low, mid, high) of Beilun estuary, China was investigated by high throughput DNA pyrosequencing of the 16S rRNA gene. The results demonstrated that bacterial communities in mangrove sediments of Beilun estuary were highly diverse. Shannon index of microbial diversity from the different intertidal zones ranged from 10.2 to 10.4. Phylogenetic analysis showed that bacterial communities from mangrove sediments were dominated by Proteobacteria, followed by Chloroflexi, Bacteroidetes, Planctomycetes and Acidobacteria. Venn diagram of OTUs distribution suggested that bacterial composition from the different intertidal zones displayed a high similarity. However, heat map and ternary plot demonstrated that each intertidal zone was inhabited by specia l dominant bacterial groups. Pyrosequencing also indicated that some potential bacteria from mangrove played an important role in organic pollutant transformation and biogeochemical transformation.
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