拟南芥VTE1过量表达可以增加维生素E含量和提高烟草植株耐盐性

维生素E在动物细胞内具有抗氧化等重要作用,但在植物体内的功能却鲜为人知.本实验利用CaMV35S启动子与来源于拟南芥的编码生育酚环化酶(TC)的cDNA(VTE1)构建的嵌合表达载体,以根癌农杆菌介导的叶盘法转化烟草W38.实验结果表明,具有卡那霉素抗性的再生植株经RT-PCR检测,得到了与阳性对照一致的495bp的目标片段;转基因植株的VE含量比对照植株高2倍左右,个别株系高达11倍.实验还发现,在耐盐性实验中转基因植株对盐的抗性明显高于野生型烟草;同时,在不同盐浓度(150、250mmol/L)胁迫下转基因植株VE含量比未转化植株增加了1.3~1.8倍,首次证明VTE1与植物耐盐性之间的关系.图7参30

Overexpression of VTE1 from Arabidopsis Resulting in High Vitamin E Accumulationand Salt Stress Tolerance Increase in Tobacco Plant~ *

Vitamin E is an essential antioxidant in the human cells, while its function in plants is unknown. In this study, tobacco W38 was transformed with a construct containing a cDNA insert encoding tocopherol cyclase (VTE1) from Arabidopsis under the control of the cauliflower mosaic virus (CaMV) 35S promoter. The transgenic tobacco plants were confirmed by RT-PCR, which yielded the same PCR product of 495 bp as the positive control. Compared to the wild type controls, up to 11 times of vitamin E were accumulated in different transgenic lines. Interestingly, the over-expression of VTE1 dramatically increased the salt stress tolerance in tobacco plants. To our knowledge, it is first reported that VTE1 can increase salt stress tolerance in plants. Fig 7, Ref 30