信号分子对高负荷UASB中ANAMMOX颗粒特性的影响

针对高负荷反应器中ANAMMOX颗粒污泥易上浮流失的问题，进行外源添加酰基高丝氨酸内酯类信号分子（AHLs）对污泥特性的影响研究.结果表明添加30mg/L辛酰基高丝氨酸内酯（C8-HSL）可对颗粒污泥沉降性能产生长期影响，能够有效控制高负荷UASB中ANAMMOX颗粒污泥上浮.仅在实验初期（0~20d）向反应器R2中添加C8-HSL，在实验进行至100d时R2中颗粒污泥B-EPS含量相比对照组R1降低15%，PN/PS值由4.22下降至2.14，同时污泥颗粒表面疏水性提高了26%，因此R2中颗粒污泥的沉降性能大幅提高（颗粒污泥密度增加了24%，沉速提高了90%）.实验进行至100d，R2未发生较明显污泥上浮现象，此时氮容积负荷NLR为12.9kg-TN/（m³·d），氮容积去除率NRR高达11.3kg-TN/（m³·d），氮去除率达88%.己酰基高丝氨酸内酯（C6-HSL）可使污泥颗粒的活性有所提高，而十二烷酰基高丝氨酸内酯（C12-HSL）则对高负荷反应器中颗粒污泥的特性没有影响.

The effects of signal molecules on the characteristics of ANAMMOX granules in high-loaded UASB reactors

A typical setback of ANAMMOX granules was their flotation when the reactor was run at high loading rates. The effects of Acylated Homoserine Lactones (AHLs) on the characteristics of ANAMMOX granules in high-loaded UASB reactors were investigated. The results showed that the exogenous addition of 30mg/LN-octanoyl-PL-homoserine lactone (C8-HSL) had long-term effects on the settleability of ANAMMOX granules and was able to control the sludge floating effectively. At the beginning of the experiment (0~20d), C8-HSL was added to the reactor R2. While compared with the control group R1, the content of B-EPS in the granular sludge in R2was decreased by 15% and the granular sludge's value of PN/PS dropped from 4.22 to 2.14 on the 100^th day. In addition, the hydrophobicity of the granular sludge in R2 was increased by 26% on the 100^th day. Therefore, the settleability of sludge granules in R2 was improved significantly (the density of the granules was increased by 24%, and the settling velocity of the granules was increased by 90%). During the 100days, no obvious granule floatation was observed in R2. On the 100^th day, the NLR was 12.9kg-TN/(m³·d) and the NRR was up to 11.3kg-TN/(m³·d) in R2. At the same time, the nitrogen removal efficiency was up to 88% in R2. C6-HSL improved the activity of the granular sludge, while C12-HSL had no effects on the characteristics of the granules in the high-loaded reactors.