德国分散式污水处理系统微生物群落结构及功能

以德国分散式污水处理系统为研究对象，通过16S rRNA测序，研究2个反应器中微生物群落结构，利用PICRUSt软件对其功能进行推演.结果表明，冬季，污水厌氧膜生物反应器（AnMBR）内温度20℃，进水COD 712mg/L时，出水可获得52%的COD平均去除率，产气率为122L/kgCOD；固体废物厌氧反应器（PSD）内温度37℃，反应器内COD 3007mg/L时，可获得374L/kgVSS的产气率.2个反应器具有相似的微生物组成，对细菌，Synerigistaceae科的相对丰度最高（AnMBR：24.0%±10.0%；PSD：11.0%±3.1%）；对古菌，Methanobacteriaceae科的相对丰度最高（AnMBR：0.6%±0.3%；PSD：13.8%±1.8%）；2个反应器的功能基因组成也相似，产甲烷都以H₂还原CO₂的通路为主.PSD反应器中H₂还原CO₂通路相关基因、F420合成相关基因、辅酶M合成相关基因的相对丰度都高于AnMBR反应器.

Microbial community structure and function in a decentralized urban water infrastructure systems in German

The microbial community structure in two bioreactors of a decentralized urban water infrastructure systems in Germany was investigated through 16S rRNA gene sequencing. The PICRUSt software was then used to deduce the function of the microbial community. In winter, the average COD removal rate reached 52% at 20℃, the influent COD was 712mg/L in the anaerobic membrane reactor (AnMBR) and the biogas production rate was 122L/kgCOD. At 37℃, the biogas production rate was 374L/kgVSS and the COD was 3007mg/L in the primary sludge digester (PSD). Similar microbial compositions were observed in both bioreactors, with Synerigistaceae having the highest relative abundance at the family level among bacteria (AnMBR:24.0%±10.0%; PSD:11.0%±3.1%) and Methanobacteriaceae having the highest relative abundance among archaea (AnMBR:0.6%±0.3%; PSD:13.8%±1.8%). The functional genomic composition was also similar in both reactors, with the H₂reductive CO₂ pathway being the main approach for methane production. The relative abundances of H₂ reductive CO₂ pathway related genes, F420synthesis related genes and coenzyme M synthesis related genes in the PSD reactor were higher than those in the AnMBR reactor.