| 精喹禾灵降解菌株Bacillus subtilis H的分离鉴定及降解特性 |
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| 引用本文： | 侯颖,李静泉,尤晓颜,王维宇,裴韬,孙军杰.精喹禾灵降解菌株Bacillus subtilis H的分离鉴定及降解特性[J].中国环境科学,2018,38(4):1466-1472. |
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| 作者姓名： | 侯颖 李静泉 尤晓颜 王维宇 裴韬 孙军杰 |
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| 作者单位： | 1.内蒙古大学生态与环境学院, 内蒙古自治区环境污染控制与废物资源化重点实验室, 内蒙古 呼和浩特 010021;2.河南科技大学食品与生物工程学院, 河南 洛阳 471023 |
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| 基金项目： | 内蒙古自治区重大基础研究开放课题（30500-515330304）；国家自然科学基金青年基金项目（31400098）；河南省科技攻关项目（172102310435）；河南科技大学人才基金项目（09001642）；河南科技大学青年科学基金项目（2013N019）；河南科技大学科研创新能力培育基金项目（2014ZCX025）；河南科技大学大学生研究训练计划（SRTP）项目（2015067） |
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| 摘 要： | 以精喹禾灵为唯一碳源，从长期受精喹禾灵污染土壤中分离筛选出一株精喹禾灵降解菌株H.通过形态观察、生理生化试验和16S rRNA基因序列分析，将菌株H鉴定为枯草芽孢杆菌（Bacillus subtilis ）.菌株H在温度为30~42℃和pH值7~9范围内，72h内对100mg/L精喹禾灵的降解率均可达95%以上.利用超高效液相色谱-质谱联用（UPLC-MS）鉴定菌株H降解精喹禾灵的产物为精喹禾灵酸.这是首次报道枯草芽孢杆菌对精喹禾灵的降解.
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| 关 键 词： | 精喹禾灵 枯草芽孢杆菌 鉴定 降解特性 |
| 收稿时间： | 2017-09-08 |
Isolation and identification of quizalofop-p-ethyl-degrading strain Bacillus subtilis H and its degradation characteristics |
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| HOU Ying,LI Jing-quan,YOU Xiao-yan,WANG Wei-yu,PEI Tao,SUN Jun-jie.Isolation and identification of quizalofop-p-ethyl-degrading strain Bacillus subtilis H and its degradation characteristics[J].China Environmental Science,2018,38(4):1466-1472. |
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| Authors: | HOU Ying LI Jing-quan YOU Xiao-yan WANG Wei-yu PEI Tao SUN Jun-jie |
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| Institution: | 1. Inner Mongolia Key Laboratory of Environmental Pollution Control & Waste Resource, School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China;
2. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China |
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| Abstract: | Strain H degrading quizalofop-p-ethyl (QE) was isolated by using QE as sole carbon source from the soil contaminated by QE for a long time. Strain H was identified as Bacillus subtilis based on morphological properties, physiological and biochemical characteristics and 16S rRNA gene analysis. The degradation rate of 100mg/L QE was over 95% within 72h when strain H was cultured at temperature of 30~42℃ and pH 7~9. The degradation product of QE was identified as quizalofop acid(QA) by UPLC-MS. This is the first report of quizalofop-p-ethyl degrading by Bacillus subtilis. |
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| Keywords: | quizalofop-p-ethyl Bacillus subtilis identification degradation characteristics |
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