嘉陵江出口段水体碱性磷酸酶活性特征

于2011~2016年间,从嘉陵江重庆主城段水域的"磷营养状态-总碱性磷酸酶活性变化-藻华"之间的相关关系入手,通过原位实验总结TAPA与ALGAE及SRP变化规律,结合碱性磷酸酶反应动力学实验确定酶活参数,探讨酶活变化在"藻华"中的生态学意义.结果表明：消落期时,SRP为藻类生长活动主要的磷源."藻华"敏感期SRP含量全年最低,为0.012~0.021mg/L;TAPA全年最高,为10.503~11.587nmol/（L·min）.特征参数V_m和米氏常数K_m值的变化规律验证了"藻华"敏感期各样点碱性磷酸酶对底物的亲合力增高,酶的水解速率有了显著提升;碱性磷酸酶在其他形态的磷转化为SRP的过程中有很大的促进作用,为藻类等微生物生长提供充足的SRP,以利于其在适宜的生境中迅速增殖.

Alkaline phosphatase activity of Jialing River estuary

This paper investigated the interrelationship between phosphorus status, change of total alkaline phosphatase activities (TAPA), and algae bloom of the Jialing River Chongqing section from 2011 to 2016. The changes of TAPA, ALGAE and SRP were summarized through in-situ experiments. The activity parameters of alkaline phosphatase were determined, and the ecological significance of enzyme activity in algal bloom was discussed. The results showed that Soluble Reactive Phosphorus (SRP) was the main source of phosphorus for algae growth. The lowest SRP detected as 0.012~0.021mg/L was observed during the critical period of algae bloom, and the highest TAPA was 10.503~11.587nmol/L/min. The variations of characteristic parameters V_m and the Michaelis~Menten constant K_m validated that alkaline phosphatase had a higher affinity for substrate and the hydrolysis rate of the enzyme increased significantly during the critical period of algae bloom. It is also found that alkaline phosphatase significantly promoted the transformation of other forms of phosphorus into SRP, thus supplying adequate SRP to the growth of microorganisms such as algae.