厌氧氨氧化耦合反硝化底物竞争抑制特性

通过连续流实验和批式实验研究了有机物和NO₂^--N对厌氧氨氧化菌和反硝化菌耦合脱氮特性的影响.在连续流实验中，保证底物NO₂^--N充足，研究了葡萄糖有机物对厌氧氨氧化颗粒污泥反应器脱氮性能的影响.当进水葡萄糖有机物的COD浓度为100mg/L时，颗粒污泥具有良好的厌氧氨氧化耦合反硝化脱氮活性，当COD浓度为200mg/L时，颗粒污泥的厌氧氨氧化耦合反硝化脱氮活性较差.当进水COD浓度分别为100，200mg/L时，反应器中颗粒污泥的厌氧氨氧化NH₄⁺-N去除活性分别为0.096，0.071kg NH₄⁺-N/（kgVSS-d），厌氧氨氧化NO₂^--N去除活性分别为0.153，0.092kg NO₂^--N/（kgVSS-d），反硝化NO₂^--N去除活性分别为0.111，0.212kg NO₂^--N/（kgVSS-d）.在批式实验中，研究了碳源种类和COD/NO₂^--N比对厌氧氨氧化耦合反硝化颗粒污泥脱氮性能的影响.控制COD/NO₂^--N比为1~4，以葡萄糖为碳源时，厌氧氨氧化菌在亚硝态的竞争过程中占据优势；以乙酸钠为碳源时，控制COD/NO₂^--N比为1~4，厌氧氨氧化菌在亚硝态的竞争过程中处于劣势.

The inhibitive characteristics by substrate competition of the anammox coupling denitrification process

The effect of organic matters and nitrite on the anaerobic ammonium oxidation (anammox)-denitrification coupling process was studied in a continuous flow reactor and batch tests. During the continuous flow experiment, the effect of glucose on the nitrogen removal performance of the anammox granules reactor was investigated with the addition of sufficient nitrite. As a result, the granules performed excellent anammox-denitrification coupling activities with the COD concentration of 100mg/L. While the COD concentration was improved to 200mg/L, the granules performed bad anammox-denitrification coupling activities. With the COD concentrations of 100 and 200mg/L, the corresponding ammonium removal activities of granules through anammox process were 0.096 and 0.071kg NH₄⁺-N/(kgVSS -d). The nitrite removal activities of granules through anammox process were 0.153 and 0.092kg NO₂^--N/(kgVSS -d). Meanwhile, the corresponding denitrification activities of the granules were 0.111 and 0.212kg NO₂^--N/(kgVSS -d). As for the batch tests, the effect of carbon sources and chemical oxygen demand (COD)/NO₂^--N ratios on the nitrogen removal performance of the anammox-denitrification coupling granules was analyzed. With the carbon source of glucose and the COD/NO₂^--N ranges of 1 to 4, the anammox bacteria performed greater nitrite competitive ability than denitrifiers. However, with the carbon source of sodium acetate and the COD/NO₂^--N ranges of 1 to 4, the anammox bacteria performed lower nitrite competitive ability than denitrifiers.