重金属污染土壤接种丛枝菌根真菌对蚕豆毒性的影响

采用盆栽实验的方法,研究了重金属(包括Cu、Zn、Pb和Cd)复合污染和接种丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)Glomus mosseae对蚕豆(Vicia faba)生长及DNA损伤的影响.结果表明,虽然接种菌根真菌对蚕豆生物量的影响并不显著,但是却显著影响植物对重金属的吸收,接种菌根真菌对蚕豆吸收4种重金属元素的作用有差异.采用单细胞凝胶电泳(single cell gel electrophoresis,SCGE)法研究接种菌根真菌对蚕豆叶片的DNA损伤的影响,与重金属吸收的结果相吻合.结果表明,接种处理可显著增加蚕豆叶片的DNA损伤程度,这与接种处理可提高植物的重金属吸收相一致.

Effects of arbuscular mycorrhizal fungi colonization on toxicity of soil contaminated by heavy metals to Vicia faba

Vicia faba was selected as a host plant in a greenhouse-pot-culture experiment to investigate the effects of arbuscular mycorrhizal fungi (AMF) colonization on the toxicity to Vicia faba induced by heavy metal contaminated soil. The results suggested that AMF colonization decreased the toxicity of heavy metals to Vicia faba. Compared with control, the P uptake was increased by AMF colonization but the biomass of mycorrhizal plants was not increased by AMF colonization. It showed large differences in uptakes of four heavy metals by mycorrhizal and nonmycorrhizal Vicia faba: AMF colonization enhanced the uptakes of Cu, Zn, Pb and Cd by Vicia faba. There existed large differences in DNA damage in the leaves of nonmycorrhizal and mycorrhizal plants detected by single cell gel electrophoresis,SCGE. The DNA damage in the leaves was increased by AMF colonization which was consistent with the increased metals uptake induced by AMF colonization.