马赛菌属Massilia neuiana异养硝化-好氧反硝化脱氮特征实验

基于马赛菌属脱氮Massilia neuiana的模式菌株PTW21进行研究，探讨其异养硝化-好氧反硝化脱氮能力。菌株PTW21具有高效异养硝化和好氧反硝化能力，对NH₄+-N和NO₂--N去除率均超过90%。同时，菌株PTW21具有同步硝化反硝化能力，且反硝化效率高于硝化效率，但当有NH₄+-N存在时，会优先利用NH₄+-N，再利用NO₂--N，存在硝化-反硝化竞争抑制现象。同步硝化反硝化时，菌株PTW21可以去除95%以上的NH₄+-N和NO₂--N。Massilia neuiana的研究丰富了异养硝化-好氧反硝化微生物的种类，也为该菌种在污水处理厂的生物强化应用提供了前期基础。

ANALYSIS ON THE HETEROTROPHIC NITRIFICATION-AEROBIC DENITRIFICATION CHARACTERISTICS OF THE NEW SPECIES OF MASSILIA NEUIANA

In this study, the heterotrophic nitrification-aerobic denitrification characteristics of strain PTW21, a typical strain of a novel nitrogen removal species Massilia neuiana, was investigated. The high efficiencies of heterotrophic nitrification and aerobic denitrification by strain PTW21 were demonstrated, which were both obtained over 90%. A higher denitrification efficiency was obtained than its nitrification efficiency in the simultaneous nitrification and denitrification (SND) by strain PTW21. However, a nitrification-denitrification competitive inhibition was observed in the presence of NH₄+-N that ammonium was preferentially consumed by strain PTW21, followed by nitrate. Totally over 95% of ammonia and nitrate nitrogen were removed by strain PTW21 in the SND process. The study on Massilia neuiana enriched the variety of heterotrophic nitrification-aerobic denitrification microbe, and established the preliminary foundation of the strain for the bio-augmentation application in sewage treatment plants.