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| 产毒微囊藻mcyA基因荧光定量PCR方法的建立 | |
| 引用本文： | 何恩奇,钮伟民,吴庆刚,周伟杰,晏丽,张银志,孙秀兰.产毒微囊藻mcyA基因荧光定量PCR方法的建立[J].环境科学与技术,2011,34(12):66-70. |
| 作者姓名： | 何恩奇 钮伟民 吴庆刚 周伟杰 晏丽 张银志 孙秀兰 |
| 作者单位： | 1. 无锡市疾病预防控制中心,江苏无锡,214023 2. 江南大学食品学院,江苏无锡,214122 3. 江南大学食品科学与技术国家重点实验室,江苏无锡,214122 |
| 基金项目： | 江苏省卫生厅科研基金项目(HD200865); 无锡市科技局指导性项目(CSZ00958) |
| 摘 要： | 针对微囊藻毒素合成酶基因mcyA探索一种适用于自然水样中微囊藻毒素产毒潜能检测的TaqMan实时荧光定量PCR方法.该方法从铜绿微囊藻FACHB-1279中的mcyA基因中扩增出目的基因片段,用TA克隆方法将基因片段插入Allel TA vector (ABP-CETAVEC020)中来制备质粒标准晶.通过不同连续稀释... |
| 关 键 词： | 铜绿微囊藻 mcyA TaqMan实时荧光定量PCR 水样 |
Establishment of TaqMan Real-time Fluorescence PCR of Gene McyA of Toxin-producing Microcystis spp |
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| HE En-qi,NIU Wei-min,WU Qing-gang,ZHOU Wei-iie,YAN Li,ZHANG Yin-zhi,SUN Xiu-lan.Establishment of TaqMan Real-time Fluorescence PCR of Gene McyA of Toxin-producing Microcystis spp[J].Environmental Science and Technology,2011,34(12):66-70. | |
| Authors: | HE En-qi NIU Wei-min WU Qing-gang ZHOU Wei-iie YAN Li ZHANG Yin-zhi SUN Xiu-lan |
| Institution: | HE En-qi1,NIU Wei-min1,WU Qing-gang1,ZHOU Wei-jie1,YAN Li2,ZHANG Yin-zhi3,SUN Xiu-lan2(1.Wuxi Center for Disease Control and Prevention,Wuxi 214023,China,2.School of Food Science and Technology,Jiangnan University,Wuxi 214122,3.Key Laboratory of Food Science and Technology,School of Food Science,China) |
| Abstract: | TaqMan fluorescent quantitative polymerase chain reaction(PCR) detection method aiming at microcystins synthase gene mcyA which can be used to detect natural water was established.Gene fragments were amplified with gene mcyA of Microsystis aeruginosa FACHB-1279.The plasmid standard product was prepared by TA clone method which inserted the purpose gene fragments into Allel TA vector.With different continuous dilutions,the standard curve of mcyA gene was successfully established.The standard curve correlatio... |
| Keywords: | Microcystis aeruginosa mcyA gene TaqMan real-time fluorescence quantitative polymerase chain reaction(PCR) water sample |
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