高效脱硫菌的筛选及其性能研究

从高硫污染的活性污泥中富集培养，分离纯化得到一株可以降解噻吩的菌株s_4，并对该菌株的形态特征进行观察。应用Design—Expert8．0．5b软件进行响应面优化实验，研究了反应时间、噻吩浓度、微生物浓度3个因素的组合对菌株s_4脱硫效果的影响，并拟合得到多元二次回归方程，得出最佳实验条件。拟合结果表明，当反应时间27．46h，噻吩浓度为1．04％，微生物浓度4．04％时，预测噻吩降解率为14．8％，通过验证得最佳条件下的降解率为14．3％，与预测值相符。

Screening of high efficient desulphurization bacteria and study on its properties

A strain of bacterium named S-4 which can degradate thiophene was enrichment cultured and se- lected from high sulfur polluted activated sludge, and the morphological characteristics of the strain were observed as well. The software Design-Expert 8.0.5b was applied to the response surface optimization experiment. And the influences of three factors which were reaction time, concentration of thiophene and concentration of microor- ganism were studied in the process of desulphurization bacterium S-4 degradation. The optimum experimental conditions and an equation were achieved by the software. The optimum reaction conditions were determined as follows： reaction time of 27.46 h, thiophene concentration of 1.04% , microorganism concentration of 4.04%. Under these conditions, the maximum theoretic thiophene degradation rate was 14.8%. After verifications, the maximum theoretic value was consistent with mean value of verification test, which was 14.3%.