| PERFORMANCE AND PERSISTENCE OF GREEN FLUORESCENT PROTEIN （gfp） MARKED AZOTOBACTER CHROOCOCCUM IN STERILIZED AND UNSTERILIZED WHEAT RHIZOSPHERIC SOIL |
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| 引用本文： | SINGH R,KUMAR V,SHARMA S,BEHL RK,SINGH BP,NARULA N.PERFORMANCE AND PERSISTENCE OF GREEN FLUORESCENT PROTEIN （gfp） MARKED AZOTOBACTER CHROOCOCCUM IN STERILIZED AND UNSTERILIZED WHEAT RHIZOSPHERIC SOIL[J].应用与环境生物学报,2005,11(6):751-755. |
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| 作者姓名： | SINGH R KUMAR V SHARMA S BEHL RK SINGH BP NARULA N |
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| 作者单位： | SINGH R（Department of Microbiology, SBS Post Graduate Institute of Biomedical Sciences & Research, Balawala, Dehradun, India）
KUMAR V（Department of Microbiology, SBS Post Graduate Institute of Biomedical Sciences & Research, Balawala, Dehradun, India）
SHARMA S（Department of Microbiology, SBS Post Graduate Institute of Biomedical Sciences & Research, Balawala, Dehradun, India）
BEHL RK（Department of Plant Breeding,CCS Haryana Agricultural University, Hisar, India）
SINGH BP（Department of Agronomy,CCS Haryana Agricultural University, Hisar, India）
NARULA N（Department of Microbiology, CCS Haryana Agricultural University, Hisar, India） |
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| 摘 要： | ThepracticaluseofAzotobacterchroococcumasbiofertilizer hasbeenincreasinglyidentifiedinrecentyears.Oftentheefficacy ofthesebacteriaisassessedonthebasisofcropresponseswithout knowingthesurvival,persistenceandcompetitiveabilityofthein troducedstrain.Inoculat…
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| 关 键 词： | 绿色荧光蛋白 固氮菌 消毒方法 小麦 |
| 修稿时间： | 2005年7月21日 |
PERFORMANCE AND PERSISTENCE OF GREEN FLUORESCENT PROTEIN (gfp) MARKED AZOTOBACTER CHROOCOCCUM IN STERILIZED AND UNSTERILIZED WHEAT RHIZOSPHERIC SOIL |
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| SINGH R,KUMAR V,SHARMA S,BEHL RK,SINGH BP,NARULA N.PERFORMANCE AND PERSISTENCE OF GREEN FLUORESCENT PROTEIN (gfp) MARKED AZOTOBACTER CHROOCOCCUM IN STERILIZED AND UNSTERILIZED WHEAT RHIZOSPHERIC SOIL[J].Chinese Journal of Applied and Environmental Biology,2005,11(6):751-755. |
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| Authors: | SINGH R KUMAR V SHARMA S BEHL RK SINGH BP NARULA N |
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| Abstract: | The persistence and performance (growth promoting potential) of green fluorescent protein (gfp) marked Azotobacter chroococcum strain ABR 4G were studied in sterilized and unsterilized wheat rhizospheric soil. The gfp was integrated via Tn 5 transposition into A. chroococcum chromosome and the resultant gfp marked colonies were identified by green fluorescent emission under UV light. The gfp was stably maintained in A. chroococcum and the gfp insertion had no apparent adverse effect on the growth promoting properties of the marked soil isolate ABR 4G. The growth promoting properties (nitrogen fixation, ammonia excretion, phosphate solubilization and IAA production) of the parent soil isolate and the gfp marked strain were found to be almost the same. All the quantitative wheat plant traits were significantly influenced by inoculation of A. chroococcum ABR 4G strain in sterilized and unsterilized soil. Inoculated bacterial counts increased gradually in wheat rhizosphere, reached maximum on 60 th d and declined on 80 th d. Fertility levels also affected survival of marked strain and the survival was comparable in sterilized and unsterilized soil. The growth promoting properties were also determined from the marked strain reisolated from wheat rhizosphere in both types of soil. Fig 1, Tab 2, Ref 22 |
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| Keywords: | green fluorescent protein (gfp) Azotobacter chroococcum wheat rhizospheric soil sterilization |
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