正交实验选择嗜碱细菌降解木质素的最优综合培养条件

在复合碳源的碱性液体培养条件下(pH≈10.5),用正交实验法对影响嗜碱木质素降解细菌6号菌株降解木质素的7种单因素培养条件进行了优化.结果表明,第1碳源用量等单因素在不同程度上对6号菌株产生的木质素降解酶Laccase和MnP以及木质素降解率都有一定促进作用.正交实验表明,其中第1碳源用量和T-80用量是显著性的影响因素,7种单因素的最佳综合水平为:37℃静置培养10d、第1碳源(蔗糖)1g/L、氮源NH₄NO₃1.2g/L、培养基70ml/250ml锥形瓶、初始pH值10.6和第2碳源接种后第4d加入、添加0.3%的表面活性剂T-80.

Optimization of Synthetic Culture Condition on Lignin Biodegradation Ability of Alkaliphilic Ligninolytic Bacteria Using Orthogonal Experimental Design

The effects of seven single culture factors on lignin biodegradation ability of alkaliphilic ligninolytic bacteria strain 6 in alkaline liquid medium (pH approximately 10.5) with compounded carbons were optimized using orthogonal experiments. The result showed the seven single culture factors promote the activities of Laccase and MnP and the degradation rate of wheat straw lignin in varying degrees. The synthetic result was analysised. The optimal synthetic condition was static culture (10 days culture period), 37 degrees C, the initial pH10.6, 1 g/L sugar (the starting carbons), wheat straw lignin (the second carbons, wheat straw powder 20 g/L, added into on the fourth day after inoculation), 1.2 g/L NH4NO3 (nitrogen), 0.3% detergent T-80, 70 ml medium/250 ml flask.