玉米秸秆发酵浸出液模拟废水发酵产氢的放大实验研究

在20L的半连续流发酵罐中，以牛粪堆肥为产氢菌源，按照玉米秸秆发酵浸出液的主要成份配制模拟废水，考察和分析了几个关键环境因素对发酵产氢的影响。结果表明，HRT、C/N、Fe2+浓度和模拟废水浓度对发酵产氢均有不同程度的影响。在本实验条件下，秸秆模拟废水的氢产量、氢浓度和产氢速率分别为11.80mol/kg、56%和8.81L/(L?d)，底物转化率大于90%，废水中COD去除率为39.40%。在整个发酵产氢过程，液相主要发酵副产物为丁酸、乙酸和丙酸以及少量的乙醇和丁醇。

Enlargement Test Studies of Bio-hydrogen Production using Artificial Wastewater of Corn Stalk Fermentation Lixivium by Mixed Culture

Conversion of artificial corn stalk wastewater, which was prepared according to the main composition of the corn stalk fermentation lixivium, into bio-hydrogen gas by mixed culture was performed in a 20 L half-continuous flow fermenter. The influences of several environmental factors on the bio-hydrogen production, such as HRT, C/N ratio, Fe2+ and artificial corn stalk wastewater concentration were discussed in the tests. The experimental results showed that HRT, C/N ratio, Fe2+ and artificial corn stalk wastewater concentration significantly affected the fermentation hydrogen production. The maximum H2 yield of 11.80 mol/kg, H2 concentration of 56% and hydrogen production rate of 8.81 L/(L x d) were obtained at HRT = 10 h, C/N = 100, Fe2+ concentration of 100 mg/L and substrate concentration of 12.5 g/L by mixed culture, respectively. In the fermentation hydrogen-producing process, the conversion efficiency of the substrate was more than 90%, and 39.40% of COD was removed from the reactor. The main by-products in the liquid phase were acetic acid, butyric acid, propionic acid and a little ethanol and butanol throughout this study.