Characterization and role of carbonic anhydrase in the calcification process of the azooxanthellate coral <Emphasis Type="Italic">Tubastrea aurea</Emphasis> |
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Authors: | Sylvie Tambutté Eric Tambutté Didier Zoccola Natacha Caminiti Severine Lotto Aurélie Moya Denis Allemand Jess Adkins |
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Institution: | (1) Centre Scientifique de Monaco, av. Saint Martin, MC, 98 000, Monaco;(2) Department of Geology and Planetary Sciences, MS100-23, Caltech, 1200 E. California Blvd., Pasadena, CA 91125, USA;(3) UMR 1112 UNSA-INRA Faculté des Sciences, Parc Valrose, B.P.71, 06108 Nice Cedex 2, France |
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Abstract: | In zooxanthellate corals, the photosynthetic fixation of carbon dioxide and the precipitation of CaCO3 are intimately linked both spatially and temporally making it difficult to study carbon transport mechanisms involved in each
pathway. When studying Tubastrea aurea, a coral devoid of zooxanthellae, we can focus on carbon transport mechanisms involved only in the calcification process.
We performed this study to characterize T. aurea carbonic anhydrase and to determine its role in the calcification process. We have shown that inhibition of tissular carbonic
anhydrase activity affects the calcification rate. We have measured the activity of this enzyme both in the tissues and in
the organix matrix extracted from the skeleton. Our results indicate that organic matrix proteins, which are synthesized by
the calcifying tissues, are not only structural proteins, but they also play a crucial catalytic role by eliminating the kinetic
barrier to interconversion of inorganic carbon at the calcification site. By immunochemistry we have demonstrated the presence
of a protein both in the tissues and in the organic matrix, which shares common features with prokaryotic carbonic anhydrases. |
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Keywords: | Carbonic anhydrase Carbon Calcification Coral Biomineralization Organic matrix |
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