生物样品中含PBDEs在内的复合污染组分的抗雌激素效应

为初步探讨电子废物拆解导致的多溴二苯醚(PBDEs)及其类似物构成的复合污染的潜在生态/健康风险,从电子废物拆解区的三黄鸡血液和肝脏样品中提取了包含PBDEs在内的复合污染组分,分别体外暴露乳腺癌MCF-7细胞和MDA-MB-231细胞6d,检测细胞增殖和雌激素靶基因pS2的mRNA表达.结果表明,包含多种PBDEs在内的复合污染组分在不产生细胞毒性的前提下,可显著抑制MCF-7细胞增殖和雌激素靶基因pS2的mRNA表达,表现出抗雌激素活性.此结果提示由电子废物拆解造成的复合污染对生物体和人体可能存在潜在的生态/健康风险.

Anti-Estrogenic Effects of Complex Extracts Containing Polybrominated Diphenyl Ethers from Biotic Samples

In order to explore preliminarily the potential risk of the complex pollutants containing polybrominated diphenyl ethers(PBDEs)from biotic samples from E-waste treating areas,the complex pollutants containing PBDEs were extracted from Sanhuang chicken samples from an E-waste recycling region.Human breast cancer MCF-7 cells and MDA-MB-231 cells were exposed to the extracts for 6d,and the cell proliferation and mRNA expression of pS2,an estrogen-target gene,were determined.Results showed that the extracts had no cytotoxic effects but suppressed significantly the cell proliferation and mRNA expression of pS2 in MCF-7 cells.The extracts exhibited anti-estrogenic activity.The results suggest that complex pollutants containing PBDEs from E-waste recycling regions have certain potential risk for animal and human health.