缺氧反硝化除磷菌驯X菌的筛选与生长条件优化

以SBR强化生物除磷装置污泥为研究对象，进行反硝化除磷菌的筛选,并用传统与现代分子生物学相结合手段确定其分类地位，同时采用响应面分析试验进行菌的生长条件优化. 结果表明:驯X菌的生理生化指标显示其具有缺氧反硝化除磷功能，根据细菌形态观察、培养特征、生理生化指标和16S rDNA测序结果，驯X菌与Escherichia coli最相似，同源性高达99.9%，因此驯X菌(Escherichia coli)是缺氧反硝化除磷菌. 由响应面分析可知，影响驯X菌生长的关键因素次序为ρ(碳源)＞pH＞ρ(氮源)，二次项中ρ(碳源)2也是唯一的显著因素，ρ(碳源)对菌的生长具有决定作用；其中碳源采用乙酸钠+葡萄糖，氮源采用硫酸铵+蛋白胨. 驯X菌的最优化生长条件:ρ(碳源)为3.48g/L，ρ(氮源)为1.22 g/L，pH为8.00，此时实测的菌浊平均值为0.732. 

Screening and Growth Condition Optimization of Anoxic Denitrifying Phosphorus Removal Bacteria Xun X

Sludge from an SBR enhanced biological phosphorus removal device was studied to screen the denitrifying phosphorus removal bacteria(DPB) Xun X.Its taxonomic status was determined by traditional and molecular biology technology.Response surface methodology was used to optimize the growth conditions of Xun X.Physiological and biochemical properties of Xun X show its anoxic denitrifying and phosphorus removal function.It is most similar to Escherichia coli,according to morphological observation,culture charact...