Improving bioavailability of phosphorous from cattle dung by using phosphatase immobilized on natural clay and nanoclay |
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Authors: | Marcela Calabi-Floody Gabriela Velásquez Liliana Gianfreda Surinder Saggar Nanthi Bolan Cornelia Rumpel María Luz Mora |
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Institution: | a Scientific and Technological Bioresource Nucleus (BIOREN-UFRO), Universidad de La Frontera, Av. Francisco Salazar 01145, Temuco, Chile b Dipartamento di Scienze del Suolo, della Pianta, dell’Ambiente e delle Produzioni Animali, Universitá di Napoli Federico II, Italy c Landcare Research, Palmerston North 4442, New Zealand d Centre for Environmental Risk Assessment and Remediation (CERAR), University of South Australia, SA 5095, Adelaide, Australia e Laboratoire de Biogéochimie et Ecologie des Milieux Continentaux (BIOEMCO, UMR Université Paris VI et XII-CNRS-INRA-IRD), Campus AgroParisTech, Thiverval-Grignon, France |
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Abstract: | The high P retention of acidic Andisols makes necessary to increase our technological approaches in pasture management in the animal system production. Here, we evaluated the clay- or nanoclay-acid phosphatase complexes for improving phosphorus mineralization from degraded cattle dung. We implemented an immobilization mechanism of acid phosphatase (AP) using natural clays (allophanic and montmorillonite) and nanoclays as support materials. Also, we evaluated the mineralization of organic P containing in decomposed cattle dung with clay- and nanoclay-AP complexes by incubation studies. Clays and nanoclays were characterized by microscopy techniques as atomic force and confocal-laser scanning microscopy. We found that these support materials stabilized AP by encapsulation. Our results showed that immobilization on allophanic or montmorillonite materials improved both the specific activity (4-48%) and the Vmax (28-38%) of AP. Moreover, the enzyme had a better performance when immobilized on clay and nanoclay from Andisol than on montmorillonite materials. Phosphorous mineralization of cattle dung was regulated by water-soluble P present in the dung and P re-adsorption on allophanic materials. However, we were able to detect a potential capacity of AP immobilized on allophanic nanoclays as the best alternative for P mineralization. Further research with initially low water-soluble P containing organic materials is required to quantify the P mineralization potential and bioavailability of P from dung. |
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Keywords: | Nanoclays Andisols Phosphorous mineralization Acid phosphatase Enzyme immobilization |
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