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| CMS高粱保持系叶绿体基因ps1A1和ps1A2片段的克隆与序列比对 | |
| 引用本文： | 孙春昀,范昌发,张福耀,牛天堂,孙毅,郭骁才.CMS高粱保持系叶绿体基因ps1A1和ps1A2片段的克隆与序列比对[J].应用与环境生物学报,2003,9(5):506-512. |
| 作者姓名： | 孙春昀 范昌发 张福耀 牛天堂 孙毅 郭骁才 |
| 作者单位： | 1. 中国科学院成都生物研究所,成都,610041 2. 山西省农业科学院高梁研究所,山西榆次,030600 3. 山西省农业科学院生物工程中心,太原,030031 |
| 基金项目： | 中国科学院生物科学与技术研究特别支持费课题 (STZ 1 1 6)，成都市科技攻关重点项目资助~~ |
| 摘 要： | 以15种包括同质异核和同核异质高粱材料的总：DNA为模板进行RAPD分析，196个随机引物中，引物Y-19扩增得到一个很有规律的差异片段SAY-192300。该片段只出现在4个保持系(B)和3个恢复系(R)中，而不出现在6个不育系(A)和2个杂种F1中，进一步对cms—Al、cms—A2两套8个材料(A／B／R／F1)的总DNA、mtDNA和cpDNA用引物Y-19进行扩增，发现片段SAY-192300仅在cpDNA得到，Southern杂交结果证实了片段SAY-192300的叶绿体来源，同时也表明在cms胞质中该片段所在区未发生插入、缺失等大的结构变异,DNA序列测定结果表明，该片段为叶绿体ps1A1和ps1A2基因的部分序列。图6表2参19。 |
| 关 键 词： | 高粱 细胞质雄性不育 序列分析 叶绿体基因 |
| 修稿时间： | 2002年12月3日 |
CLONING ANG SEQUENCE ANALYSIS OF ps1A1 AND ps1A2 GENES AMPLIFIED SPECIFICALLY FROM THE CHLOROPLAST DNA OF MAINTAINER OF CMS SORGHUM |
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| SUN Chunyun,FAN Changfa,ZHANG Fuyao,NIU Tiantang,SUN Yi & GUO Xiaocai.CLONING ANG SEQUENCE ANALYSIS OF ps1A1 AND ps1A2 GENES AMPLIFIED SPECIFICALLY FROM THE CHLOROPLAST DNA OF MAINTAINER OF CMS SORGHUM[J].Chinese Journal of Applied and Environmental Biology,2003,9(5):506-512. | |
| Authors: | SUN Chunyun FAN Changfa ZHANG Fuyao NIU Tiantang SUN Yi & GUO Xiaocai |
| Institution: | SUN Chunyun,FAN Changfa,ZHANG Fuyao1,NIU Tiantang2,SUN Yi2 & GUO Xiaocai ** |
| Abstract: | The total DNAs from 15 allocytoplasmic or allonuclear sorghum species were analyzed by RAPD analysis, and a fragment SAY-19 2300 was amplified specifically from total DNAs of seven sorghum cultivars including 4 maintainers and 3 restorers with fertile (N) cytoplasm. PCR assays showed that this fragment was amplified from chloroplast (cp) DNA. The total DNA, mitochondrial (mt) DNA, and cpDNA were digested with EcoRI+HindIII, and hybridized with fragment SAY-19 2300 as probe. The hybridization signals appeared both in total DNA and cpDNA but not in mtDNA. No obviously and regularly polymorphic hybridization signals between the N- cytoplasm and sterile (S) cytoplasm were observed. Sequence analysis revealed this newly cloned fragment contained a portion of ps1A1 (1-2139bp) and ps1A2 (2165-2447bp) genes. Fig 6, Tab 2, Ref 19 |
| Keywords: | Sorghum bicolor (L ) Moench cytoplasmic male sterility ps1A1 and ps1A2 genes sequence analysis |
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