| 壬基酚降解酶的固定化及特性研究 |
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| 引用本文： | 张美珍,徐文韬,张小凡,邓国志,何义亮.壬基酚降解酶的固定化及特性研究[J].环境科学与技术,2013(3):1-4. |
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| 作者姓名： | 张美珍 徐文韬 张小凡 邓国志 何义亮 |
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| 作者单位： | 上海交通大学环境科学与工程学院 |
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| 基金项目： | 国家科学自然基金资助项目(50978161);“863”水专项(2008ZX07211-002) |
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| 摘 要： | 用海藻酸钠包埋法对壬基酚(NP)降解酶进行了固定化研究,并对固定化酶的最适反应温度和最适pH、及其稳定性进行了探讨。实验结果表明,用3%海藻酸钠、2%CaCl2溶液固定化的酶活力最高;固定化NP降解酶的最适反应温度为35℃,最适pH为7.0,其热稳定性和pH稳定性都有一定程度的提高。。
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| 关 键 词： | 壬基酚 生物降解 固定化酶 海藻酸钠 酶活性 |
Immobilization and Characterization of Nonylphenol-degrading Enzyme |
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| ZHANG Mei-zhen,XU Wen-tao,ZHANG Xiao-fan,DENG Guo-zhi,HE Yi-liang.Immobilization and Characterization of Nonylphenol-degrading Enzyme[J].Environmental Science and Technology,2013(3):1-4. |
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| Authors: | ZHANG Mei-zhen XU Wen-tao ZHANG Xiao-fan DENG Guo-zhi HE Yi-liang |
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| Institution: | (School of Environmental Science and Engineering,Shanghai Jiaotong University,Shanghai 200240,China) |
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| Abstract: | Nonylphenol-degrading enzyme was prepared in the laboratory scale and the optimum condition regarding temperature,pH and stability of the immobilized enzyme was studied.The experimental result showed that immobilization with 3% sodium alginate and 2% CaCl2 generated the highest enzymatic activity,and the optimum reaction temperature of immobilization was 35 ℃,the optimum pH 7.0.In addition,the thermal stability and pH stability of the enzyme could be upgraded to some extent. |
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| Keywords: | nonylphenol biodegradation immobilized enzyme sodium alginate enzyme activity |
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