微生物发酵产物中溶藻物质的提取方法

微生物溶藻是藻类水华控制的研究热点，但目前仍缺乏去除微生物发酵产物中营养物质的方法学研究，对野外水体采集的微囊藻的溶藻活性研究也较少见。比较了不同极性溶剂对溶藻物质的固液萃取效率，研究了分子筛层析对溶藻物质的纯化效果，并测定了各提纯步骤的中间产物对野外水体采集的铜绿微囊藻的溶藻效果。结果表明，在溶藻活性基本保持稳定的前提下，固液萃取串联分子筛层析技术对TOC、TN和TP的总去除率平均高达97%，其中，固液萃取的分级去除率平均高达90%，而分子筛层析的分级去除率平均为70%，且分子筛层析后的产物对野外水体采集的铜绿微囊藻的去除率稳定在60%左右。结果说明，固液萃取与分子筛层析技术可以作为溶藻物质工业化提取与实验室纯化的重要方法。

Extraction method of algal-lysing substance from microbial fermentation products

Algal-lysing microorganisms are a research focus for the control of algal blooms. However, methodological studies investigating the removal of nutrients from fermentation products are rare. Moreover, previous studies have seldom used Microcystis sampled from the field to test algal-lysing effects. This research compared the solid-liquid extraction efficiency of different polar solvents to algal-lysing substances, studied the effect of molecular sieve chromatography on purification, and measured the algal-lysing effect on Microcystis sampled from the field. The results showed that under conditions of stable algae-lysing activity, the overall removal rate of TOC, TN, and TP was as high as 97%, and the single-step removal rate of solid-liquid extraction was 90%, while the single-step removal rate of molecular sieve chromatography was 70%. After molecular sieve chromatography, the rate of algal lysing of Microcystis sampled from the field was about 60%. These results indicate that solid-liquid extraction and molecular sieve chromatography could be used to purify algal-lysing fermentation products in industrialization and laboratory studies.