Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches |
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Authors: | Decai Jin Ping Wang Zhihui Bai Xinxin Wang Hong Peng Rong Qi Zhisheng Yu and Guoqiang Zhuang |
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Institution: | 1. Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.;Graduate University of Chinese Academy of Sciences, Beijing 100049, China 2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China 3. Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. 4. School of Chemical Engineering, The University of Queensland, Brisbane, Queensland 4072, Australia |
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Abstract: | The bacterial community of a bulking sludge from a municipal wastewater treatment plant with anoxic-anaerobic-oxic process was investigated by combination of cultivation and 16S rRNA gene clone library analysis for understanding the causes of bulking. A total of 28 species were obtained from 63 isolates collected from six culture media. The most cultivable species belonged to γ-Proteobacteria including Klebsiella sp., Pseudomonas sp., Aeromonas sp. and Acinetobacter sp. Further analysis of these strains by repetitive sequence based on polymerase chain reaction (rep-PCR) technology showed that rep-PCR yielded discriminatory banding patterns within the same genus using REP and BOX primer sets. While the culture-independent assessment revealed that β-Proteobacteria was the dominant group in the bulking sample. Sequence analysis revealed that the highest proportion (14.7%) of operational taxonomic units was 98% similar to Candidatus Accumulibacter phosphatis, which is used to remove phosphorous from wastewater. Our results indicated that combining différent approaches can produce complementary information, thus generate a more accurate view of microbial community in bulking sludge. |
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Keywords: | bulking sludge cultivable bacteria rep-PCR 16S rRNA gene clone library |
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