固定化无花果曲霉对弱酸猩红FG脱色研究

采用海藻酸钙法固定不同状态无花果曲霉(Aspergillus ficuum),形成活菌固定化小球和死菌固定化小球。并探讨了活菌固定化小球和死菌固定化小球在不同培养时间、温度、pH、染料浓度等条件下对偶氮染料弱酸猩红FG的脱色效果。试验结果表明:在温度为33℃、pH5.0、转速150r/min的条件下,经48h固定化无花果曲霉可达最佳脱色效果,脱色率达90％以上。固定化活菌的脱色效果明显优于死菌。固定化活菌脱色动力学试验表明:活菌固定化小球对不同初始浓度的弱酸猩红FG的脱色遵循零级反应。活菌固定化小球经3次脱色后能重复利用,脱色率仍达87.2％。

Study on Decolorization of Acidity Scarlet FG by Immobilized Aspergillus ficcum

Aspergillus ficcum was immobilized with sodium alginate, and formed in different states: live and heat inactivated and alginate immobilized cells. This paper explored the effect of decolorization of different states immobilized cell under conditions such as temperature, pH, culture time. The results indicated that immobilized live cell has its best effect of high decolorizing rate over 90% at pH=5.0, 33℃ and 48h. Comparing the effect of decolorization of different states cells, apparently immobilized live cell has advantage over heat inactivated cells. The paper also studied the change of dye concentration with time under different dye concentration. The results showed that decolorization of Acidity Scarlet on Aspergillus ficcum agree with zero-order reaction model. The immobilized live cells could be reused after decolorizing for third times, and decolorization rate could also reach 87.2%.